Evaluation of uterine receptivity after gonadotropin releasing hormone agonist administration as an oocyte maturation trigger: a rodent model

• Published in: Scientific reports Vol. 9; no. 1; pp. 12519 - 10
• Main Authors: Ezoe, Kenji, Murata, Nana, Yabuuchi, Akiko, Kobayashi, Tamotsu, Kato, Keiichi
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 29.08.2019; Nature Publishing Group UK
• Subjects: Acetic acid; Agonists; Animals; Artificial insemination; Buserelin - administration & dosage; Buserelin - adverse effects; Chorionic gonadotropin; Chorionic Gonadotropin - metabolism; Disease Models, Animal; Drug Evaluation, Preclinical; Embryo Implantation - drug effects; Embryo Transfer; Endometrium; Endometrium - drug effects; Endometrium - metabolism; Epithelium; Estradiol - metabolism; Female; Fertilization; Fertilization in Vitro; Gonadotropin-releasing hormone; Gonadotropin-Releasing Hormone - agonists; Gonadotropins; Humans; Implantation; In vitro fertilization; Infertility, Female - drug therapy; Infertility, Female - genetics; Infertility, Female - metabolism; Infertility, Female - physiopathology; Male; Menopause; Mice; Mice, Inbred ICR; Oocytes - cytology; Oocytes - drug effects; Oogenesis - drug effects; Ovary - drug effects; Ovary - metabolism; Pituitary (anterior); Pregnancy; Pregnancy Rate; Progesterone; Progesterone - metabolism; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Receptors, Progesterone - genetics; Receptors, Progesterone - metabolism; Secretion; Steroidogenesis; Uterus; Uterus - drug effects; Uterus - physiopathology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-48918-3

In natural cycle or minimal stimulation cycle IVF, buserelin acetate (buserelin), a gonadotropin-releasing hormone agonist, is often used as a maturation trigger; however, its effect on pregnancy outcomes remains unclear. Therefore, in the present study, we compared uterine receptivity in buserelin-administered mice with that in human chorionic gonadotropin (hCG)-administered mice during the peri-implantation period. Implantation, decidualisation, and term-pregnancy were impaired following hCG, but not buserelin administration. hCG stimulated the synthesis and secretion of progesterone and oestradiol, whereas ovarian steroidogenesis in the buserelin-treated group was comparable with that in the control group. Furthermore, similar to the observation in controls, the buserelin-treated group exhibited activation of progesterone receptor signalling and inhibition of oestrogen receptor signalling in the endometrial epithelium on the day of implantation. However, epithelial progesterone signalling was not detected, and a high expression of genes downstream to oestrogen was observed on day 4 following hCG administration. These results suggest that buserelin administration does not impact uterine receptivity as it did not affect ovarian steroidogenesis and endometrial steroid signalling. Therefore, buserelin is preferred as an oocyte maturation trigger to optimise uterine receptivity during treatments involving timed intercourse, intrauterine insemination, or fresh embryo transfer following in vitro fertilisation.