Spectroscopic signatures of the T to R conformational transition in the insulin hexamer

• Published in: The Journal of biological chemistry Vol. 264; no. 32; pp. 19081 - 19085
• Main Authors: Roy, M, Brader, M L, Lee, R W, Kaarsholm, N C, Hansen, J F, Dunn, M F
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 15.11.1989; American Society for Biochemistry and Molecular Biology
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Insulin - metabolism; Kinetics; Macromolecular Substances; Magnetic Resonance Spectroscopy - methods; Models, Molecular; N.M.R; Protein Conformation; Protein hormones. Growth factors. Cytokines; Proteins; Spectrophotometry - methods; Protein hormone; Absorption spectrometry; Pancreatic hormone; Cobalt Ions; Polymer; NMR spectrometry; Insulin; Spatial structure; Conformational transition
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(19)47269-4

The cobalt(II)-substituted human insulin hexamer has been shown to undergo the phenol-induced T6 to R6 structural transition in solution. The accompanying octahedral to tetrahedral change in ligand field geometry of the cobalt ions results in dramatic changes in the visible region of the electronic spectrum and thus represents a useful spectroscopic method for studying the T to R transition. Changes in the Co2+ spectral envelope show that the aqua ligand associated with each tetrahedral Co2+ center can be replaced by SCN-, CN-, OCN-, N3-, Cl-, and NO2-. 19F NMR experiments show that the binding of m-trifluorocresol stabilizes the R6 state of zinc insulin. The chemical shift and line broadening of the CF3 singlet, which occur due to binding, provide a useful probe of the T6 to R6 transition. Due to the appearance of new resonances in the aromatic region, the 500 MHz 1H NMR spectrum of the phenol-induced R6 hexamer is readily distinguishable from that of the T6 form. 1H NMR studies show that phenol induces the T6 to R6 transition, both in the (GlnB13)6(Zn2+)2 hexamer and in the metal-free GlnB13 species; we conclude that metal binding is not a prerequisite for formation of the R state in this mutant.