NADH reduction of nitroaromatics as a probe for residual ferric form high-spin in a cytochrome P450

• Published in: Biochimica et biophysica acta Vol. 1866; no. 1; pp. 126 - 133
• Main Authors: Pochapsky, Thomas C., Wong, Nathan, Zhuang, Yihao, Futcher, Jeffrey, Pandelia, Maria-Eirini, Teitz, Drew R., Colthart, Allison M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2018
• Subjects: Acetophenones - chemistry; Acetophenones - metabolism; Amino Acid Motifs; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding Sites; Biocatalysis; biodiversity; biotechnology; camphor; Camphor - chemistry; Camphor - metabolism; Camphor 5-Monooxygenase - chemistry; Camphor 5-Monooxygenase - genetics; Camphor 5-Monooxygenase - metabolism; Cloning, Molecular; cytochrome P-450; Electron paramagnetic resonance; electron paramagnetic resonance spectroscopy; Electron Spin Resonance Spectroscopy; Escherichia coli - genetics; Escherichia coli - metabolism; Ferric Compounds - chemistry; Gene Expression; Heme - chemistry; Heme - metabolism; Heme protein; iron; Kinetics; Models, Molecular; mutants; mutation; NAD (coenzyme); NAD - chemistry; NAD - metabolism; nitroaromatic compounds; Oxidation-Reduction; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Substrate recognition; Substrate Specificity; Heme protein; Substrate recognition; Electron paramagnetic resonance
• ISSN: 1570-9639; 0006-3002; 1878-1454; 1878-2434
• DOI: 10.1016/j.bbapap.2017.04.003

The existence of a substrate-sensitive equilibrium between high spin (S=5/2) and low spin (S=1/2) ferric iron is a well-established phenomenon in the cytochrome P450 (CYP) superfamily, although its origins are still a subject of discussion. A series of mutations that strongly perturb the spin state equilibrium in the camphor hydroxylase CYP101A1 were recently described (Colthart et al., Sci. Rep. 6, 22035 (2016)). Wild type CYP101A1 as well as some CYP101A1 mutants are herein shown to be capable of catalyzing the reduction of nitroacetophenones by NADH to the corresponding anilino compounds (nitroreductase or NRase activity). The distinguishing characteristic between those mutants that catalyze the reduction and those that cannot appears to be the extent to which residual high spin form exists in the absence of the native substrate d-camphor, with those showing the largest spin state shifts upon camphor binding also exhibiting NRase activity. Optical and EPR spectroscopy was used to further examine these phenomena. These results suggest that reduction of nitroaromatics may provide a useful probe of residual high spin states in the CYP superfamily. This article is part of a Special Issue entitled: Cytochrome P450 biodiversity and biotechnology, edited by Erika Plettner, Gianfranco Gilardi, Luet Wong, Vlada Urlacher, Jared Goldstone. •Some variants of CYP101A1 catalyze the reduction of nitroacetophenones to the corresponding anilines (NRAse activity).•Mutants that do not catalyze the reduction exhibit <50% high spin form upon binding of native substrate.•NRAse activity is proposed as a probe for residual high spin in cytochromes P450.