2-benzothiazoleacetonitrile based two-photon fluorescent probe for hydrazine and its bio-imaging and environmental applications

• Published in: Scientific reports Vol. 7; no. 1; pp. 1530 - 8
• Main Authors: Wang, Jian-Yong, Liu, Zhan-Rong, Ren, Mingguang, Lin, Weiying
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 08.05.2017; Nature Publishing Group UK; Nature Portfolio
• Subjects: Acetonitriles - chemical synthesis; Acetonitriles - chemistry; Animals; Benzothiazoles - chemical synthesis; Benzothiazoles - chemistry; Cytotoxicity; Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent indicators; Gases; HeLa Cells; Humans; Hydrazine; Hydrazines - analysis; Hydrogen-Ion Concentration; Mass spectrometry; Mice; Molecular Imaging; Photons; Rats; Scientific imaging; Spectrometry, Fluorescence
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-01656-w

A novel turn-on two-photon fluorescent probe NS-N H was developed with the 2-benzothiazoleacetonitrile as a new recognition site for the detection of hydrazine (N H ). The two-photon probe exhibited favorable properties including high selectivity, low cytotoxicity and almost 16-fold fluorescence enhancement in the presence of N H in solution. The probe could be used to image hydrazine in the living cells. Notably, we also used the two-photon fluorescent probe to image hydrazine in the tissue imaging for the first time. Furthermore, by the way of probe-loaded TLC plate, we further monitored vapor of hydrazine. Therefore, the novel two-photon probe is expected to be employed to detect N H in biosamples and environmental pollution and the new recognition site will be widely applied to construct fluorescent probes for the detection of N H .