Analysing uncharted transcriptomes with SAGE

• Published in: Trends in genetics Vol. 16; no. 10; pp. 423 - 425
• Main Authors: Velculescu, Victor E., Vogelstein, Bert, Kinzler, Kenneth W.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2000; Elsevier Science
• Subjects: Animals; Biological and medical sciences; Databases, Factual; Diverse techniques; DNA, Complementary - biosynthesis; DNA, Complementary - genetics; DNA, Neoplasm - genetics; Expressed Sequence Tags; Fundamental and applied biological sciences. Psychology; Gene expression; Gene expression databases; Gene Expression Profiling; Humans; Internet; Microchemistry - methods; Molecular and cellular biology; Moloney murine leukemia virus - enzymology; mRNA; Neoplasms - genetics; Polymerase Chain Reaction; RNA-Directed DNA Polymerase - metabolism; SAGE; Sequence Homology, Nucleic Acid; serial analysis of gene expression; Small cell populations; Transcription, Genetic; Transcriptome; transcriptosomes; Transcriptome; Genetics; Small cell populations; Gene expression databases; SAGE; mRNA; Gene expression; Techniques & methods; Bioinformatics; Analysis method; Gene; Disease; Serial analysis of gene expression; Genome; Application
• ISSN: 0168-9525
• DOI: 10.1016/S0168-9525(00)02114-4

Methods of comprehensive gene expression analysis have traditionally been limited to analysing bulk tissue or millions of cells. New modifications of serial analysis of gene expression (SAGE) have now permitted the analysis of gene expression in cell subpopulations or microanatomic structures, providing access to unexplored transcriptomes of normal and disease biology.