Effect of black grape juice against heart damage from acute gamma TBI in rats

• Published in: Molecules (Basel, Switzerland) Vol. 18; no. 10; pp. 12154 - 12167
• Main Authors: de Freitas, Robson Borba, Boligon, Aline Augusti, Rovani, Bruno Tomazele, Piana, Mariana, de Brum, Thiele Faccim, da Silva Jesus, Roberta, Rother, Fagner Chagas, Alves, Nelson Mendes, Teixeira da Rocha, João Batista, Athayde, Margareth Linde, Barrio, Juan Pablo, de Andrade, Edson Ramos, de Freitas Bauerman, Liliane
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.09.2013; MDPI
• Subjects: acute radiation syndrome; Animals; Antioxidants - chemistry; Antioxidants - pharmacology; Antioxidants - therapeutic use; black grape juice; Dehydrogenases; Flavonoids; Flavonoids - chemistry; Flavonoids - pharmacology; Flavonoids - therapeutic use; Free radicals; Fruit - chemistry; Gamma Rays; Health sciences; Heart; Heart - drug effects; Heart - radiation effects; HPLC-DAD; L-Lactate Dehydrogenase - metabolism; lactate dehydrogenase; Lipid Peroxidation; Lipids; Male; Malondialdehyde - metabolism; Metabolites; oxidative damage; phenolics; Phenols - chemistry; Phenols - pharmacology; Phenols - therapeutic use; Physiology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Radiation; Radiation Injuries, Experimental - drug therapy; Radiation Injuries, Experimental - metabolism; Radiation-Protective Agents - chemistry; Radiation-Protective Agents - pharmacology; Radiation-Protective Agents - therapeutic use; Rats; Rats, Wistar; Thiobarbituric Acid Reactive Substances - metabolism; Vitis - chemistry; Whole-Body Irradiation; Brazil; Rio de Janeiro Brazil
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules181012154

The aim of this study was to evaluate the potential positive effect of black grape juice (BGJ) on lipid peroxidation considering Total Body Irradiation (TBI) in Wistar rats. As a potential feasible means of evaluation in situ, blood serum lactate dehydrogenase (LDH) levels were evaluated as a marker for heart damage from acute radiation syndrome (ARS). Twenty rats were divided into four groups, two of them being irradiated by gamma-rays from a Co-60 source. Animals were treated by gavage with 2 mL per day of BGJ or placebo for one week before and 4 days after 6 Gy whole body gamma-irradiation, when they were euthanasiated. LDH on serum and lipid peroxidation on heart tissue were evaluated. High concentration of metabolites from lipid peroxidation in heart, and high LDH level on serum were found only in gamma-irradiated group given placebo, mainly at the first 24 h after radiation. Phytochemical analysis of BGJ was performed by determining total phenolics, flavonoids, and tannins followed by a high-performance liquid chromatography (HPLC/DAD) analysis, which showed resveratrol as the major constituent. Results suggest that BGJ is a good protective candidate compound against heart damage from ARS and its effects suggest its use as a radiomodifier.