Biomimetic Cationic Nanoparticles Based on Silica: Optimizing Bilayer Deposition from Lipid Films

• Published in: Biomimetics (Basel, Switzerland) Vol. 2; no. 4; p. 20
• Main Authors: Ribeiro, Rodrigo T, Braga, Victor H A, Carmona-Ribeiro, Ana M
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.10.2017; MDPI
• Subjects: Adsorption; AEROSIL OX-50; Atmospheric aerosols; cationic bilayer fragments; Cholera; colloidal stability; dioctadecyldimethylammonium bromide; elemental analysis for in situ adsorption; films of cationic lipids; Ligands; Lipid bilayers; Lipids; N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride; Nanoparticles; optimal bilayer adsorption from films; Peptides; Proteins; Silica; Silicon wafers; Transmission electron microscopy; Zeta potential; United States > US; colloidal stability; optimal bilayer adsorption from films; elemental analysis for in situ adsorption; N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride; AEROSIL OX-50; films of cationic lipids; cationic bilayer fragments; dioctadecyldimethylammonium bromide
• ISSN: 2313-7673; 2313-7673
• DOI: 10.3390/biomimetics2040020

The optimization of bilayer coverage on particles is important for a variety of biomedical applications, such as drug, vaccine, and genetic material delivery. This work aims at optimizing the deposition of cationic bilayers on silica over a range of experimental conditions for the intervening medium and two different assemblies for the cationic lipid, namely, lipid films or pre-formed lipid bilayer fragments. The lipid adsorption on silica in situ over a range of added lipid concentrations was determined from elemental analysis of carbon, hydrogen, and nitrogen and related to the colloidal stability, sizing, zeta potential, and polydispersity of the silica/lipid nanoparticles. Superior bilayer deposition took place from lipid films, whereas adsorption from pre-formed bilayer fragments yielded limiting adsorption below the levels expected for bilayer adsorption.