Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR

• Published in: Scientific reports Vol. 7; no. 1; pp. 4774 - 7
• Main Authors: Chen, Rong, Wang, Jiayu, Yuan, Yi, Deng, Yun, Lai, Xianrong, Du, Feng, Dong, Juan, Huang, Xin, Cui, Xin, Tang, Zhuo
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 06.07.2017; Nature Publishing Group UK; Nature Portfolio
• Subjects: Biomedical materials; Crocus - genetics; Deoxyribonucleic acid; DNA; DNA - analysis; Food industry; Herbal medicine; Plants, Medicinal - genetics; Polymerase chain reaction; Quality Control; Real-Time Polymerase Chain Reaction - methods; Reproducibility of Results; Species Specificity
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-05083-9

What's on the label is not what's in the bottle, from food products to herbal medicinal products (HMPs), economically-motivated biomaterials adulteration is a long-term problem affecting the food and drug industry. Accurate identification of the biomaterial ingredients in processed commodities is highly desirable. In this field, DNA-based techniques have proved to be powerful tools to overcome qualitative challenges. However, is it possible to quantify the weight of biological materials with PCR? Therefore, a basic scientific question needs to be answered: what's the relationship between DNA content and the mass of biological materials? Is DNA content directly proportional to the mass of biological materials as most of the researchers previously thought? In this study, we firstly found that there exists a linear relation between DNA contents and the weight of biomaterials indeed when the analytical practices are fully controlled. In this case, the mass of targeted biomaterials in the highly processed commercial products can also be calculated by quantifying the species-specific DNA through classic real-time PCR with a good reproducibility.