A modular DNA origami-based enzyme cascade nanoreactor

In this communication, we present a nanoscale reactor assembled from tuneable and spatially addressable tubular DNA origami units. We can controllably combine separate origami units equipped with glucose oxidase (GOx) and horseradish peroxidase (HRP), and demonstrate efficient GOx/HRP enzyme cascade...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 51; no. 25; pp. 5351 - 5354
Main Authors Linko, Veikko, Eerikäinen, Marika, Kostiainen, Mauri A
Format Journal Article
LanguageEnglish
Published England 28.03.2015
Subjects
Deoxyribonucleic acid
DNA - chemistry
DNA - metabolism
Enzymes
Glucose oxidase
Glucose Oxidase - chemistry
Glucose Oxidase - metabolism
Horseradish Peroxidase - chemistry
Horseradish Peroxidase - metabolism
Modularity
Nanostructure
Nanostructures - chemistry
Particle Size
Peroxidase
Reactors
Surface Properties
Tubes
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Summary:In this communication, we present a nanoscale reactor assembled from tuneable and spatially addressable tubular DNA origami units. We can controllably combine separate origami units equipped with glucose oxidase (GOx) and horseradish peroxidase (HRP), and demonstrate efficient GOx/HRP enzyme cascade reaction inside the tube. The reactor could be utilized as a nanoscale diagnostic tool, and modularity of the proposed system would further enable more complex reactions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc08472a