Effect of dibutyryl cyclic adenosine 3',5'-monophosphate on morphological features and biological markers of human salivary myoepithelial cell line in culture

• Published in: Cancer investigation Vol. 10; no. 2; p. 111
• Main Authors: Kawamata, H, Azuma, M, Yanagawa, T, Yura, Y, Yoshida, H, Furumoto, N, Sato, M
• Format: Journal Article
• Language: English
• Published: England 1992
• Subjects: Analysis of Variance; Bucladesine - pharmacology; Catecholamines - metabolism; Cell Line; Clone Cells; Cyclic AMP - metabolism; Epithelium - drug effects; Epithelium - pathology; Fluorescent Antibody Technique; Humans; Phosphopyruvate Hydratase - metabolism; Salivary Gland Neoplasms; Synaptophysin - metabolism; Tumor Cells, Cultured
• ISSN: 0735-7907
• DOI: 10.3109/07357909209032772

We have found the emergence of myoepithelial cells (HSG-AZA1) in neoplastic human salivary intercalated duct cell line HSG in culture after treatment with 5-azacytidine. When HSG-ZAZ1 cells were cultured in the presence of N6,O2-dibutyryl cyclic adenosine 3',5'-monophosphate (dB-cAMP), they formed long cytoplasmic processes which were densely packed with ample microfibrils in addition to microtubule bundles. The expression of neuron-specific enolase, synaptophysin, and catecholamine as well as neurofilaments in the treated HSG-AZA1 cells was found by the immunofluorescence staining technique, immunoblotting, immunoelectron microscopy, or catecholamine fluorescence. Both the anchorage-independent and anchorage-dependent growths of HSG-AZA1 cells were suppressed in the presence of dB-cAMP. After the removal of dB-cAMP from the culture, the treated cells returned rapidly to the phenotype and growth rate of the untreated cells. These findings indicate that reversible differentiation into the neuron-like cells of HSG-AZA1 cells occurs in growth medium containing dB-cAMP.