Comparative mechanisms of cancer cell migration through 3D matrix and physiological microtracks

• Published in: American Journal of Physiology: Cell Physiology Vol. 308; no. 6; pp. C436 - C447
• Main Authors: Carey, Shawn P, Rahman, Aniqua, Kraning-Rush, Casey M, Romero, Bethsabe, Somasegar, Sahana, Torre, Olivia M, Williams, Rebecca M, Reinhart-King, Cynthia A
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 15.03.2015
• Series: Physical Biology of Cancer
• Subjects: Actins - metabolism; Actomyosin - metabolism; Animals; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Call for Papers; Cancer; Cell Adhesion; Cell adhesion & migration; Cell Line, Tumor; Cell Movement; Cell Shape; Collagen - metabolism; Comparative analysis; Cytoskeleton; Cytoskeleton - metabolism; Extracellular Matrix - metabolism; Extracellular Matrix - pathology; Female; Humans; Integrin beta1 - metabolism; Mammary Neoplasms, Experimental - genetics; Mammary Neoplasms, Experimental - metabolism; Mammary Neoplasms, Experimental - pathology; Matrix; Mechanotransduction, Cellular; Mice, Inbred NOD; Mice, Transgenic; Neoplasm Invasiveness; Polymerization; Time Factors; Tumor Microenvironment; migration mechanisms; microtracks; tumor invasion
• ISSN: 0363-6143; 1522-1563
• DOI: 10.1152/ajpcell.00225.2014

Tumor cell invasion through the stromal extracellular matrix (ECM) is a key feature of cancer metastasis, and understanding the cellular mechanisms of invasive migration is critical to the development of effective diagnostic and therapeutic strategies. Since cancer cell migration is highly adaptable to physiochemical properties of the ECM, it is critical to define these migration mechanisms in a context-specific manner. Although extensive work has characterized cancer cell migration in two- and three-dimensional (3D) matrix environments, the migration program employed by cells to move through native and cell-derived microtracks within the stromal ECM remains unclear. We previously reported the development of an in vitro model of patterned type I collagen microtracks that enable matrix metalloproteinase-independent microtrack migration. Here we show that collagen microtracks closely resemble channel-like gaps in native mammary stroma ECM and examine the extracellular and intracellular mechanisms underlying microtrack migration. Cell-matrix mechanocoupling, while critical for migration through 3D matrix, is not necessary for microtrack migration. Instead, cytoskeletal dynamics, including actin polymerization, cortical tension, and microtubule turnover, enable persistent, polarized migration through physiological microtracks. These results indicate that tumor cells employ context-specific mechanisms to migrate and suggest that selective targeting of cytoskeletal dynamics, but not adhesion, proteolysis, or cell traction forces, may effectively inhibit cancer cell migration through preformed matrix microtracks within the tumor stroma.