Comparison of microbial communities involved in souring and corrosion in offshore and onshore oil production facilities in Nigeria

• Published in: Journal of industrial microbiology & biotechnology Vol. 41; no. 4; pp. 665 - 678
• Main Authors: Okoro, Chuma, Smith, Seun, Chiejina, Leo, Lumactud, Rhea, An, Dongshan, Park, Hyung Soo, Voordouw, Johanna, Lomans, Bart P, Voordouw, Gerrit
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.04.2014; Springer Berlin Heidelberg; Springer; Oxford University Press
• Subjects: Archaea - classification; Archaea - isolation & purification; Archaea - metabolism; Bacteria; Bacteria - classification; Bacteria - isolation & purification; Bacteria - metabolism; Biochemistry; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; calcium nitrate; Chemical analysis; Community composition; Community involvement; community structure; Corrosion; Crude oil; Environmental Microbiology; Fundamental and applied biological sciences. Psychology; Genetic Engineering; Groundwater; Groundwater - chemistry; Groundwater - microbiology; Hydrocarbons; Injection; Inorganic Chemistry; iron; Life Sciences; Methane; methanogens; Microbial activity; microbial communities; Microbial corrosion; Microbiology; Nigeria; Nitrates; Oil and Gas Fields - microbiology; Oil fields; Petroleum production; Pipelines; Production capacity; Seawater; Seawater - chemistry; Seawater - microbiology; steel; Studies; Sulfate reduction; sulfate-reducing bacteria; Sulfates; Sulfates - metabolism; Sulfides - metabolism; Temperature; Water analysis; Water sampling; Nigeria; Sulfide; Oil field; Sulfate-reducing bacteria; Corrosion; Souring; Methanogen; Sulfides; Methanogenic bacteria; Production; Bacterial corrosion; Microbial community; Comparative study
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-014-1401-z

Samples were obtained from the Obigbo field, located onshore in the Niger delta, Nigeria, from which oil is produced by injection of low-sulfate groundwater, as well as from the offshore Bonga field from which oil is produced by injection of high-sulfate (2,200 ppm) seawater, amended with 45 ppm of calcium nitrate to limit reservoir souring. Despite low concentrations of sulfate (0–7 ppm) and nitrate (0 ppm), sulfate-reducing bacteria (SRB) and heterotrophic nitrate-reducing bacteria (NRB) were present in samples from the Obigbo field. Biologically active deposits (BADs), scraped from corrosion-failed sections of a water- and of an oil-transporting pipeline (both Obigbo), had high counts of SRB and high sulfate and ferrous iron concentrations. Analysis of microbial community composition by pyrosequencing indicated anaerobic, methanogenic hydrocarbon degradation to be a dominant process in all samples from the Obigbo field, including the BADs. Samples from the Bonga field also had significant activity of SRB, as well as of heterotrophic and of sulfide-oxidizing NRB. Microbial community analysis indicated high proportions of potentially thermophilic NRB and near-absence of microbes active in methanogenic hydrocarbon degradation. Anaerobic incubation of Bonga samples with steel coupons gave moderate general corrosion rates of 0.045–0.049 mm/year, whereas near-zero general corrosion rates (0.001–0.002 mm/year) were observed with Obigbo water samples. Hence, methanogens may contribute to corrosion at Obigbo, but the low general corrosion rates cannot explain the reasons for pipeline failures in the Niger delta. A focus of future work should be on understanding the role of BADs in enhancing under-deposit pitting corrosion.