CID12261165, a flavonoid compound as antibacterial agents against quinolone-resistant Staphylococcus aureus

• Published in: Scientific reports Vol. 13; no. 1; p. 1725
• Main Authors: Morimoto, Yuh, Aiba, Yoshifumi, Miyanaga, Kazuhiko, Hishinuma, Tomomi, Cui, Longzhu, Baba, Tadashi, Hiramatsu, Keiichi
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 31.01.2023; Nature Publishing Group UK; Nature Portfolio
• Subjects: Amino acids; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antimicrobial activity; Antimicrobial agents; Apigenin; Deoxyribonucleic acid; DNA; DNA Gyrase; DNA topoisomerase; Drug Resistance, Bacterial; Flavonoids; Flavonoids - pharmacology; Fluoroquinolones - pharmacology; Kaempferol; Metabolites; Minimum inhibitory concentration; Penicillin; Quercetin; Secondary metabolites; Staphylococcus aureus; Staphylococcus aureus - drug effects; Strains (organisms); Supercoiling
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-28859-8

Flavonoids are plant-produced secondary metabolites that are found ubiquitously. We have previously reported that apigenin, a class of flavonoid, has unique antimicrobial activity against Staphylococcus aureus (S. aureus), one of the major human pathogens. Apigenin inhibited fluoroquinolone-resistant S. aureus with DNA gyrase harboring the quinolone-resistant S84L mutation but did not inhibit wild-type DNA gyrase. In this study, we describe five flavonoids, quercetin, luteolin, kaempferol, baicalein, and commercially available CID12261165, that show similar antimicrobial activity against fluoroquinolone-resistant S. aureus. Among them, CID12261165 was the most effective with MIC values of ≤ 4 mg/L against quinolone-resistant S. aureus strains. In vitro DNA cleavage and supercoiling assays demonstrated inhibitory activity of CID12261165 against mutated DNA gyrase, whereas activity against wild-type DNA gyrase was not observed. CID12261165 also inhibited quinolone-resistant Enterococci with an MIC value of 8 mg/L. While fluoroquinolone-resistant amino acid replacements can improve the fitness of bacterial cells, it is unknown why quinolone-susceptible S. aureus strains were predominant before the introduction of fluoroquinolone. The present study discusses the current discrepancies in the interpretation of antimicrobial activities of flavonoids, as well as the possible reasons for the preservation of wild-type DNA gyrase wherein the environmental flavonoids cannot be ignored.