Evaluation of different strains of Saccharomyces cerevisiae for ethanol production from high-amylopectin BRS AG rice (Oryza sativa L.)

• Published in: Scientific reports Vol. 12; no. 1; p. 2122
• Main Authors: Almeida, Isabela C, Pacheco, Thályta F, Machado, Fabricio, Gonçalves, Sílvia B
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 08.02.2022; Nature Publishing Group UK; Nature Portfolio
• Subjects: Amylopectin; Amylopectin - metabolism; Biofuels; Cultivars; Ethanol; Ethanol - metabolism; Fermentation; Oryza - metabolism; Oryza sativa; Rice; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Strains (organisms); Yeast
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-06245-0

Ethanol is the main biofuel produced by fermentation route and the search for new feedstocks to produce fuel ethanol is still a great challenge. This work aims to compare the ethanol production from a new irrigated rice cultivar BRS AG to the conventional cultivar BRS PAMPA applied in Brazil. Six different commercial strains of Saccharomyces cerevisiae (BG-1, CAT-1, FT-858, JP-1, PE-2, and SA-1) were applied in fermentation reactions. Fermentations performed with BRS PAMPA rice revealed that the highest yields were achieved with strain SA-1, corresponding to 93.0% of the theoretical maximum and final ethanol concentration of 58.92 g L , and with CAT-1, a yield of 92.7% and final ethanol concentration of 58.93 g L . For the fermentations with BRS AG rice, the highest yields were obtained with strain FT-858, exhibiting a 89.6% yield and final ethanol concentration of 62.45 g L , and with CAT-1, 87.9% yield and final ethanol concentration of 61.25 g L were achieved. The most appropriate microorganism for ethanol production using BRS PAMPA rice and BRS AG rice was CAT-1. Comparatively, the ethanol yield and productivity using BRS AG were higher than those observed for BRS PAMPA for all strains, except for PE-2 and SA-1 that led to very similar results. The experimental results showed that the giant rice BRS AG is an excellent feedstock for fuel ethanol production in lowland fields.