Isolation and RNA sequence analysis of cytochrome b mutants resistant to funiculosin, a center i inhibitor of the mitochondrial ubiquinol-cytochrome c reductase in Saccharomyces cerevisiae

• Published in: FEBS letters Vol. 263; no. 1; pp. 93 - 98
• Main Authors: di Rago, J.-P., Perea, J., Colson, A.-M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 09.04.1990; Elsevier
• Subjects: Alleles; Amino Acid Sequence; Animals; Anthraquinones - pharmacology; Antifungal Agents - pharmacology; Biological and medical sciences; Cytochrome b Group - genetics; Drug Resistance, Microbial - genetics; Electron Transport Complex III - antagonists & inhibitors; Fundamental and applied biological sciences. Psychology; Funiculosin resistance; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Intracellular Membranes - metabolism; Intracellular Membranes - ultrastructure; Kinetics; Membrane protein folding; Microbiology; Mitochondria - metabolism; Mitochondria - ultrastructure; Mitochondrial cytochrome b; Molecular Sequence Data; Mutation; Mutational analysis; Mycology; NAD - metabolism; Oxidation-Reduction; Protein Conformation; RNA sequencing; RNA, Fungal - genetics; RNA, Fungal - isolation & purification; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Sequence Homology, Nucleic Acid; RNA sequencing; Funiculosin resistance; Membrane protein folding; Mutational analysis; Saccharomyces cerevisiae; Mitochondrial cytochrome b; Fungi; Hemoprotein; Mitochondria; Nucleotide sequence; Microorganism culture; Enzyme inhibitor; Ascomycetes; Mutation; Molecular cloning; Thallophyta
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(90)80713-S

Funiculosin is a well-known inhibitor of the mitochondrial respiratory chain, probably acting at the ubiquinone reducing site or center i of QH 2 cytochrome c reductase. We report here the isolation, mapping and RNA sequence analysis of yeast apo-cytochrome b mutants resistant to this inhibitor. Funiculosin-resistance was found to be conferred, in 4 independent isolates, upon replacement of a leucine residue by phenylalanine in position 198 of the cytochrome b polypeptide chain.