Functional diversification of the dehydrin gene family in apple and its contribution to cold acclimation during dormancy

Dehydrins (DHN) are proteins involved in plant adaptive responses to abiotic stresses, mainly dehydration. Several studies in perennial crops have linked bud dormancy progression, a process characterized by the inability to initiate growth from meristems under favorable conditions, with DHN gene exp...

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Published inPhysiologia plantarum Vol. 155; no. 3; pp. 315 - 329
Main Authors Falavigna, Vítor da Silveira, Miotto, Yohanna Evelyn, Porto, Diogo Denardi, Anzanello, Rafael, Santos, Henrique Pessoa dos, Fialho, Flávio Bello, Margis-Pinheiro, Márcia, Pasquali, Giancarlo, Revers, Luís Fernando
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.11.2015
Wiley Subscription Services, Inc
Subjects
Acclimatization - genetics
Brazil
Cold Temperature
Evolution, Molecular
Gene expression
Gene Expression Regulation, Plant
Malus - genetics
Malus - physiology
Molecular Sequence Data
Multigene Family
Phylogeny
Plant Dormancy - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Real-Time Polymerase Chain Reaction
Brazil
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Summary:Dehydrins (DHN) are proteins involved in plant adaptive responses to abiotic stresses, mainly dehydration. Several studies in perennial crops have linked bud dormancy progression, a process characterized by the inability to initiate growth from meristems under favorable conditions, with DHN gene expression. However, an in‐depth characterization of DHNs during bud dormancy progression is still missing. An extensive in silico characterization of the apple DHN gene family was performed. Additionally, we used five different experiments that generated samples with different dormancy status, including genotypes with contrasting dormancy traits, to analyze how DHN genes are being regulated during bud dormancy progression in apple by real‐time quantitative polymerase chain reaction (RT‐qPCR). Duplication events took place in the diversification of apple DHN family. Additionally, MdDHN genes presented tissue‐ and bud dormant‐specific expression patterns. Our results indicate that MdDHN genes are highly divergent in function, with overlapping levels, and that their expressions are fine‐tuned by the environment during the dormancy process in apple.
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Table S1. Sampling dates and developmental stages of the different tissues and organs harvested.Table S2. Sampling dates, corresponding season and chilling hours accumulated by closed terminal buds harvested in 2009 and 2010.Table S3. Accession codes of DHN genes used in the phylogenetic analysis.Table S4. Primers employed in RT-qPCR studies.Fig. S1. Sequence alignment between genomic and RACE sequences of MdDHN11.Fig. S2. Significantly enriched motifs of MdDHN deduced proteins identified with the meme software.Fig. S3. PCR analysis of MdDHN12 in 'Gala Baigent' apple trees.Fig. S4. Endodormancy analysis and relative expression levels of MdDHN1-11 genes in apple twigs exposed to cold.Fig. S5. Endodormancy analysis and MdDHN1-11 relative expression in 'Royal Gala' twigs exposed to cold and growth permissive temperatures.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0031-9317
1399-3054
DOI:10.1111/ppl.12338