Acyl-CoA binding domain containing 3 (ACBD3) recruits the protein phosphatase PPM1L to ER–Golgi membrane contact sites

• Published in: FEBS letters Vol. 586; no. 19; pp. 3024 - 3029
• Main Authors: Shinoda, Yasuharu, Fujita, Kohsuke, Saito, Satoko, Matsui, Hiroyuki, Kanto, Yusuke, Nagaura, Yuko, Fukunaga, Kohji, Tamura, Shinri, Kobayashi, Takayasu
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 21.09.2012
• Subjects: ACBD3; acyl-CoA binding domain containing 3; Adaptor Proteins, Signal Transducing - chemistry; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Binding Sites; CERT; endoplasmic reticulum; Endoplasmic Reticulum - metabolism; FYCO1; FYVE and coiled-coil domain-containing protein 1; GOLD; GOLD domain; Golgi Apparatus - metabolism; Golgi dynamics; HEK293 Cells; HeLa Cells; Humans; Membrane Proteins - chemistry; Membrane Proteins - genetics; Membrane Proteins - metabolism; metal-dependent protein phosphatase 1L; Models, Biological; Models, Molecular; Phosphoprotein Phosphatases - chemistry; Phosphoprotein Phosphatases - genetics; Phosphoprotein Phosphatases - metabolism; PPM1L; Protein Interaction Domains and Motifs; Protein Transport; Protein-Serine-Threonine Kinases - chemistry; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Signal Transduction; Two-Hybrid System Techniques; Vesicular Transport Proteins - chemistry; Vesicular Transport Proteins - genetics; Vesicular Transport Proteins - metabolism; GOLD; FYCO1; PPM1L; GOLD domain; CERT; ACBD3; ER
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2012.06.050

► Acyl-CoA binding domain containing 3 (ACBD3) is an interacting partner of PPM1L. ► This association is mediated by a GOLD (Golgi dynamics) domain in ACBD3. ► ACBD3 recruits PPM1L to ER–Golgi membrane contact sites. ► ACBD3 plays a pivotal role in ceramide transport regulation. The metal-dependent protein phosphatase family (PPM) governs a number of signaling pathways. PPM1L, originally identified as a negative regulator of stress-activated protein kinase signaling, was recently shown to be involved in the regulation of ceramide trafficking at ER–Golgi membrane contact sites. Here, we identified acyl-CoA binding domain containing 3 (ACBD3) as an interacting partner of PPM1L. We showed that this association, which recruits PPM1L to ER–Golgi membrane contact sites, is mediated by a GOLD (Golgi dynamics) domain in ACBD3. These results suggested that ACBD3 plays a pivotal role in ceramide transport regulation at the ER–Golgi interface. ACBD3 and PPM1Lcolocalize by fluorescence microscopy (View interaction) FYCO1physically interacts with PPM1L by pull down (View interaction) SEC14L2physically interacts with PPM1L by pull down (View interaction) ACBD3physically interacts with PPM1L by pull down (View interaction) SEC14L1physically interacts with PPM1L by pull down (View interaction) PPM1Lphysically interacts with ACBD3 by two hybrid (View interaction)