Calcium-induced acetylcholine release and intramembrane particle occurrence in proteoliposomes equipped with mediatophore

• Published in: Biology of the cell Vol. 74; no. 2; pp. 225 - 230
• Main Authors: Brochier, Guy, Gulik-Krzywicki, Tadeusz, Lesbats, Bernard, Dedieu, Jean-Claude, Israel, Maurice
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier SAS 1992; Blackwell Publishing Ltd
• Subjects: Acetylcholine - metabolism; acetylcholine release; Animals; Calcium - metabolism; Electrophoresis, Polyacrylamide Gel; Freeze Fracturing; intramembrane particle; Kinetics; liposome; Liposomes - metabolism; mediatophore; Nerve Tissue Proteins - metabolism; Proteolipids - metabolism; Proteolipids - ultrastructure; Torpedo; acetylcholine release; mediatophore; intramembrane particle; liposome
• ISSN: 0248-4900; 1768-322X
• DOI: 10.1016/0248-4900(92)90029-Z

Proteoliposomes obtained from the mediatophore, a purified Torpedo electric organ nerve terminals protein, and endogenous lipids were used for a study of calcium-induced release of acetylcholine and freeze-fracture electron microscopy. Large intramembrane particles were induced by the influx of calcium into proteoliposomes, as previously observed for synaptosomes or stimulated electric organ nerve terminals. The involvement of mediatophore in a calcium dependent acetylcholine translocation seems therefore to be related to the occurrence of a category of intramembrane particles in the course of the release process.