Molecular detection and genomic characterization of Torque teno sus virus 1 and 2 from domestic pigs in central China

• Published in: Virus genes Vol. 46; no. 3; pp. 479 - 486
• Main Authors: Li, Kun, Wang, Lin-Qing, Wu, Yu-Yang, Chao, An-Jun, Lu, Quan-Wei, Wei, Zhan-Yong, Cui, Bao-An, Chen, Hong-Ying
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01.06.2013; Springer US; Springer Nature B.V
• Subjects: 5' Untranslated Regions; Amino Acid Motifs; Animal Structures - virology; Animals; Biomedical and Life Sciences; Biomedicine; blood; Blood - virology; China; Circovirus - genetics; Circovirus - isolation & purification; Cluster Analysis; DNA, Viral - chemistry; DNA, Viral - genetics; genome; Genome, Viral; Medical Microbiology; Molecular Sequence Data; nucleotides; Open Reading Frames; Phylogeny; Plant Sciences; Porcine Postweaning Multisystemic Wasting Syndrome - virology; sequence analysis; Sequence Analysis, DNA; Sus scrofa; Swine; TATA Box; Virology; viruses; China; Pigs; Molecular detection; Torque teno sus virus; Sequence analysis; China
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1007/s11262-013-0897-z

In the present study, Torque teno sus viruses (TTSuVs) were detected in tissue and blood samples obtained from domestic pigs in central China, and complete genomes of TTSuVs were characterized. A total of three tissue samples (3/20, 15 %) from post-weaning multisystemic wasting syndrome-affected pigs and 30 blood samples (30/40, 75 %) from healthy pigs were positive for Torque teno sus virus 1 (TTSuV1) and/or 2 (TTSuV2). Two TTSuV strains (TTV1Hn54 and TTV2Hn93) comprising 2,794 and 2,875 nucleotides, respectively, each had four open reading frames (ORFs) and the untranslated region with TATA box and GC-rich region. Genomic sequence of TTV2Hn93 strain was unique in length compared with other TTSuV2 genomic sequences. Interestingly, three rolling-circle replication (RCR) motif-IIIs (YXXK) which were located at amino acid (aa) position 166–169, 328–331, and 379–382, respectively, were found in the ORF1 of TTV1Hn54. Two RCR motif-IIIs (YXXK) at the aa position 105–108 and 480–483 respectively, were also identified in the ORF1 of TTV2Hn93. Phylogenetic tree based on complete genomes showed that TTV1Hn54 strain was designated into type TTSuV1b and had a slight high sequence identity of 91 % with the Canada strain (JQ120664). TTV2Hn93 strain was classified into subtype TTSuV2d and shared the highest identity (97 %) with the Spain strain (GU570207).