Comparison of electrochemiluminescence and ELISA methods in the detection of blood borne pathogens in Gabon

• Published in: Asian Pacific journal of tropical biomedicine Vol. 7; no. 9; pp. 805 - 808
• Main Authors: Bisseye, Cyrille, Ndong, Jophrette Mireille Ntsame, Matoumba, Anicet Mouity, Bengone, Calixte, Migolet, Guy Mouelet, Nagalo, Bolni Marius
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2017; Department of Hematology/Oncology, Mayo Clinic Scottsdale, AZ, USA; Laboratory of Molecular and Cellular Biology, University of Sciences and Techniques of Masuku(USTM), P.O.Box 943,Franceville, Gabon%National Blood Transfusion Center, P.O.Box 13895, Libreville, Gabon%Laboratory of Molecular and Cellular Biology, University of Sciences and Techniques of Masuku(USTM), P.O.Box 943,Franceville, Gabon; Wolters Kluwer Medknow Publications
• Subjects: Blood donors; Cobas 6000 e601; EVOLIS BioRad; Kappa coefficient; EVOLIS BioRad; Blood donors; Cobas 6000 e601; Kappa coefficient
• ISSN: 2221-1691; 2588-9222
• DOI: 10.1016/j.apjtb.2017.08.008

Objective: To assess the performances of Cobas 6000 e601 and EVOLIS Bio Rad in the detection of HIV, HBV and HCV in blood donors in Libreville(Gabon).Methods: A cross-sectional investigation was conducted in July 2017 in a total of 2 000 blood donors recruited at the National Blood transfusion Center, Libreville Gabon.Among them, 363 donors were selected to compare the performances of COBAS 6000 e601(electro-chemiluminescence) and EVOLIS Bio Rad in detecting HIV, HBV and HCV using Cohen’s kappa coefficient.Results: Both methods yielded similar results for the detection of HIV and HBs Ag. A very good agreement of 93.39% and an excellent agreement of 98.90% were obtained for the detection of HIV and Hbs Ag, with kappa values of 0.80 and 0.98, respectively. The observed agreement of 91.86% was found for the detection of HCV, which gave a fair agreement between the two methods with kappa = 0.33.Conclusions: The two evaluation methods showed a similar performance in the detection of HIV, HBV. However, given the high rate of intra and inter-genotypes recombination known for HIV and HBV, more robust techniques of detection such as polymerase chain reaction should be used to prevent post-transfusion contaminations.