Novel Burkholderiales 23S rRNA Genes Identified in Ileal Biopsy Samples from Children: Preliminary Evidence that a Subtype Is Associated with Perianal Crohn's Disease

• Published in: Journal of Clinical Microbiology Vol. 48; no. 5; pp. 1939 - 1942
• Main Authors: Sim, W.H, Wagner, J, Cameron, D.J, Catto-Smith, A.G, Bishop, R.F, Kirkwood, C.D
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.05.2010; American Society for Microbiology (ASM)
• Subjects: Adolescent; Bacteriology; Biological and medical sciences; Biopsy; Burkholderia - classification; Burkholderia - genetics; Burkholderia - isolation & purification; Child; Child, Preschool; Cluster Analysis; Crohn Disease - microbiology; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Humans; Ileum - microbiology; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 23S - genetics; Sequence Analysis, DNA; Human; Crohn disease; Biopsy; Ribosomal DNA; Digestive diseases; Intestinal disease; 23S-RNA; Child; Subtype; Inflammatory disease
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.02261-09

A novel family of Burkholderiales bacteria was identified in ileal biopsy specimens from children presenting with symptoms of inflammatory bowel disease. A molecular subtyping approach based on sequencing of a variable region of the bacteria's 23S rRNA genes identified three variants. Pilot analysis identified one variant to be significantly associated with perianal Crohn's disease.