protective effect of rosmarinic acid on hyperthermia-induced C2C12 muscle cells damage

• Published in: Molecular biology reports Vol. 41; no. 8; pp. 5525 - 5531
• Main Authors: Chen, Kun-Lin, Li, Hui-Xia, Xu, Xing-Lian, Zhou, Guang-Hong
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.08.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; animal tissues; Animals; Antioxidants; Antioxidants - pharmacology; Apoptosis; Apoptosis - drug effects; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Biomedical and Life Sciences; Caspase 3 - genetics; Caspase 3 - metabolism; caspase-3; Cell Line; Cell Survival - drug effects; cell viability; Cellular biology; Cinnamates - pharmacology; cultured cells; Depsides - pharmacology; gene expression; health services; heat stress; Heat-Shock Response; Histology; Hot Temperature; HSP72 Heat-Shock Proteins - genetics; HSP72 Heat-Shock Proteins - metabolism; Life Sciences; malondialdehyde; Malondialdehyde - metabolism; messenger RNA; Mice; Molecular biology; Morphology; Muscle Cells - cytology; Muscle Cells - drug effects; Muscle Cells - metabolism; Muscular system; myocytes; Oxidative Stress - drug effects; protective effect; reactive oxygen species; Reactive Oxygen Species - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rosmarinic Acid; Stress response; superoxide dismutase; Superoxide Dismutase - metabolism; temperature; Rosmarinic acid; Heat stress; C2C12 cells; Apoptosis and damage
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-014-3429-6

High temperature will cause animal tissues or cells damage. Rosmarinic acid (RA) is a good antioxidant and health care product, but the roles of RA in muscle cells damage and the mechanisms which caused by high temperature is still unknown. In this study, the roles of RA on hyperthermia-induced apoptosis and damage of C2C12 muscle cells were investigated. C2C12 cells were cultured in medium with different concentration (0, 25, 50, 100 µM) RA and treated in 42 °C high temperature to induce cellular apoptosis and damage. Then, these cells were analyzed effect of different dose of RA on cells apoptosis and damage. The results indicated that RA has protective effect on heat-stress induced cellular damage, and the cells have the higher cell viability at the dose of 50 µM RA by MTT assay. Hochest33342/PI double staining showed that the cellular apoptosis of C2C12 cells were decreased in the presence of selected 50 µM RA. Malondialdehyde formation and reactive oxygen species levels were also decreased significantly, but cellular superoxide dismutase activity was increased significantly in the presence of RA even in the condition of 42 °C. Meanwhile, Caspase-3 mRNA expression, Caspase-3 activity, and Bax/Bcl-2 ratio were reduced significantly, but the mRNA expression of Hsp72 was increased significantly in those hyperthermia-induced C2C12 cells in the presence of 50 µM RA. Taken together, the results at least discovered that RA has protective effects on hyperthermia-induced cellular apoptosis and damage of muscle cells by change the expression of stress-genes and increasing intracellular antioxidant capability.