Biomimetic Organization: Octapeptide Self-Assembly into Nanotubes of Viral Capsid-like Dimension

The controlled self-assembly of complex molecules into well defined hierarchical structures is a promising route for fabricating nanostructures. These nanoscale structures can be realized by naturally occurring proteins such as tobacco mosaic virus, capsid proteins, tubulin, actin, etc. Here, we rep...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 100; no. 18; pp. 10258 - 10262
Main Authors Valéry, Céline, Paternostre, Maïté, Robert, Bruno, Gulik-Krzywicki, Thaddée, Narayanan, Theyencheri, Dedieu, Jean-Claude, Keller, Gérard, Torres, Maria-Luisa, Cherif-Cheikh, Roland, Calvo, Pilar, Artzner, Franck
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 02.09.2003
National Acad Sciences
Subjects
Acetates
Biological Sciences
Biomimetics
Biophysics
Capsid - chemistry
Disulfides
Electron density
Hydrogen bonds
Models, Molecular
Molecular structure
Molecules
Nanotubes
Peptides
Peptides, Cyclic - chemistry
Protein Conformation
Protein Structure, Secondary
Self assembly
Somatostatin - analogs & derivatives
Somatostatin - chemistry
Vibration
Wave diffraction
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Summary:The controlled self-assembly of complex molecules into well defined hierarchical structures is a promising route for fabricating nanostructures. These nanoscale structures can be realized by naturally occurring proteins such as tobacco mosaic virus, capsid proteins, tubulin, actin, etc. Here, we report a simple alternative method based on self-assembling nanotubes formed by a synthetic therapeutic octapeptide, Lanreotide in water. We used a multidisciplinary approach involving optical and electron microscopies, vibrational spectroscopies, and small and wide angle x-ray scattering to elucidate the hierarchy of structures exhibited by this system. The results revealed the hexagonal packing of nanotubes, and high degree of monodispersity in the tube diameter (244 Å) and wall thickness (≈18 Å). Moreover, the diameter is tunable by suitable modifications in the molecular structure. The self-assembly of the nanotubes occurs through the association of β-sheets driven by amphiphilicity and a systematic aromatic/aliphatic side chain segregation. This original and simple system is a unique example for the study of complex self-assembling processes generated by de novo molecules or amyloid peptides.
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To whom correspondence should be addressed. E-mail: franck.artzner@cep.u-psud.fr.
Abbreviations: SAXS, small angle x-ray scattering; WAXS, wide angle x-ray scattering.
This paper was submitted directly (Track II) to the PNAS office.
M.P. and F.A. contributed equally to the work.
Edited by Daniel Branton, Harvard University, Cambridge, MA, and approved July 2, 2003
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1730609100