Fish anesthesia: effects of the essential oils of Hesperozygis ringens and Lippia alba on the biochemistry and physiology of silver catfish (Rhamdia quelen)

• Published in: Fish physiology and biochemistry Vol. 40; no. 3; pp. 701 - 714
• Main Authors: Toni, Cândida, Becker, Alexssandro Geferson, Simões, Larissa Novaes, Pinheiro, Carlos Garrido, de Lima Silva, Lenise, Heinzmann, Berta Maria, Caron, Braulio Otomar, Baldisserotto, Bernardo
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.06.2014; Springer Netherlands; Springer Nature B.V
• Subjects: alanine transaminase; Anesthesia; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; aspartate transaminase; Biochemistry; Biomedical and Life Sciences; blood glucose; catfish; Catfishes; essential oils; Freshwater & Marine Ecology; Histology; Lamiaceae - chemistry; Life Sciences; Lippia - chemistry; Lippia alba; Morphology; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; potassium; Rhamdia quelen; sodium; Zoology; Energy metabolism; Ionoregulation; Ventilatory rate; Anesthetics; Recovery
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-013-9877-4

The anesthetic activities of the essential oils (EOs) of Hesperozygis ringens (EOHR) and Lippia alba (EOLA) and their effects in silver catfish (Rhamdia quelen) after anesthesia and recovery were investigated. Fish (32.19 ± 1.24 g) were submitted to one of the following treatments for each EO: basal group, control, or anesthesia (150, 300, or 450 μL L⁻¹ EO). After that the anesthesia was induced or simulated and the biometric measurements were completed, fish were transferred to anesthetic-free aquaria to allow for recovery. Fish were sampled at 0, 15, 30, 60, and 240 min after recovery. At time 0 of recovery, the ventilatory rate was lower in the groups anesthetized with either EO. In comparison with the basal group, control fish showed an increase in plasma glucose, aspartate aminotransferase (AST), and Na⁺ levels and a reduction in Na⁺/K⁺-ATPase activity at 0 min of recovery. Plasma levels of ammonia and Na⁺ were lower in the fish anesthetized with EOLA (450 μL L⁻¹) and EOHR (all concentrations), respectively, than in the control fish. Additionally, lactate, AST, alanine aminotransferase, K⁺ plasma levels, and gill Na⁺/K⁺-ATPase and H⁺-ATPase activities were higher in the fish anesthetized with either EOHR or EOLA than in the control fish. The EOs promoted slight changes in silver catfish that enabled both an adaptive response and the recovery of most of the measured parameters after 240 min regardless of concentration or EO that was used. These findings support the use of EOHR and EOLA as anesthetics for fish.