Characterization of the fine specificity of bovine CD8 T-Cell responses to defined antigens from the protozoan parasite Theileria parva
Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated by CD8 T cells. Six antigens targeted by CD8 T cells from T. parva-immune cattle of different major histocompatibility complex (MHC) genotypes have been identified, raising the prospect of deve...
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Published in | Infection and Immunity Vol. 76; no. 2; pp. 685 - 694 |
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Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Society for Microbiology
01.02.2008
American Society for Microbiology (ASM) |
Subjects | |
Online Access | Get full text |
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Summary: | Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated by CD8 T cells. Six antigens targeted by CD8 T cells from T. parva-immune cattle of different major histocompatibility complex (MHC) genotypes have been identified, raising the prospect of developing a subunit vaccine. To facilitate further dissection of the specificity of protective CD8 T-cell responses and to assist in the assessment of responses to vaccination, we set out to identify the epitopes recognized in these T. parva antigens and their MHC restriction elements. Nine epitopes in six T. parva antigens, together with their respective MHC restriction elements, were successfully identified. Five of the cytotoxic-T-lymphocyte epitopes were found to be restricted by products of previously described alleles, and four were restricted by four novel restriction elements. Analyses of CD8 T-cell responses to five of the epitopes in groups of cattle carrying the defined restriction elements and immunized with live parasites demonstrated that, with one exception, the epitopes were consistently recognized by animals of the respective genotypes. The analysis of responses was extended to animals immunized with multiple antigens delivered in separate vaccine constructs. Specific CD8 T-cell responses were detected in 19 of 24 immunized cattle. All responder cattle mounted responses specific for antigens for which they carried an identified restriction element. By contrast, only 8 of 19 responder cattle displayed a response to antigens for which they did not carry an identified restriction element. These data demonstrate that the identified antigens are inherently dominant in animals with the corresponding MHC genotypes. |
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Bibliography: | L73 7714 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Department of Cardiology and Angiology, Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany. Editor: J. F. Urban, Jr. Corresponding author. Present address: Virology Department, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 44 (0)1932 357 298. Fax: 44 (0)1932 357 239. E-mail: s.graham@vla.defra.gsi.gov.uk Present address: World Organization for Animal Health (OIE), Sanseido Building, 4F, 2-4-10 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan. Present address: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, P.O. Box 1527, Heraklion 711 10, Crete, Greece. Present address: Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, United Kingdom. Present address: Vaxine Pty. Ltd., Department of Endocrinology and Diabetes, Flinders Medical Centre, Bedford Park, South Australia 5042, Australia. |
ISSN: | 0019-9567 1098-5522 |
DOI: | 10.1128/IAI.01244-07 |