Preventing further misuse of the ELISA technique and misinterpretation of serological antibody assay data

Abstract The indirect ELISA is a widely utilized method to assay serum antibodies. However, a common and critical problem when analyzing serum antibodies is the disregard for the background noise reaction caused by the hydrophobic binding of immunoglobulin components in serum components to plastic....

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Bibliographic Details
Published inVaccine Vol. 34; no. 39; pp. 4643 - 4644
Main Authors Terato, Kuniaki, Do, Christopher, Chang, Jessica, Waritani, Takaki
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 07.09.2016
Elsevier Limited
Subjects
Allergy and Immunology
Antibodies - blood
Antibody
Antigens
Background noise reaction
Collagen
Competition
Dilution
E coli
ELISA
Enzyme-Linked Immunosorbent Assay - methods
False positive reaction
False Positive Reactions
Human serum
Humans
Immunoglobulins
Medical research
Methods
Noise
Plastics - chemistry
Proteins
Serologic Tests - methods
Signal-To-Noise Ratio
Studies
Human serum
Antibody
Background noise reaction
ELISA
False positive reaction
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Summary:Abstract The indirect ELISA is a widely utilized method to assay serum antibodies. However, a common and critical problem when analyzing serum antibodies is the disregard for the background noise reaction caused by the hydrophobic binding of immunoglobulin components in serum components to plastic. Unfortunately, current blocking agents cannot prevent this background noise reaction. To prevent further misuse of the ELISA technique, it is important to openly discuss the fundamental problems involved in the ELISA system.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2016.08.007