Estrogen receptors in stromal and epithelial fractions of the ventral prostate of rats

The cytosol from rat ventral prostates contains two estrogen-binding components that can be separated in a glycerol gradient: a high-affinity (KD = 4.2 X 10(-10) M), low-capacity (110 fmole/mg DNA) species that runs in the 8S region of the gradient, and a lower-affinity (KD = 2.4 X 10(-9) M), higher...

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Bibliographic Details
Published inArchives of andrology Vol. 15; no. 2-3; p. 143
Main Authors Purvis, K, Mørkås, L, Rui, H, Attramadal, A
Format Journal Article
LanguageEnglish
Published England 1985
Subjects
Aging
Animals
Connective Tissue - analysis
Cytosol - analysis
Epithelial Cells
Epithelium - analysis
Estradiol - pharmacology
Female
Male
Prostate - analysis
Prostate - cytology
Prostate - ultrastructure
Rats
Rats, Inbred Strains
Receptors, Estradiol - metabolism
Receptors, Estrogen - analysis
Time Factors
Uterus - cytology
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Summary:The cytosol from rat ventral prostates contains two estrogen-binding components that can be separated in a glycerol gradient: a high-affinity (KD = 4.2 X 10(-10) M), low-capacity (110 fmole/mg DNA) species that runs in the 8S region of the gradient, and a lower-affinity (KD = 2.4 X 10(-9) M), higher-capacity (410 fmole/mg DNA) 4S protein. Separation of prostate tissue into stromal- and epithelial-enriched fractions was associated with the disappearance of the latter component. When compared per milligram DNA stromal- and epithelial-enriched fractions contained similar amounts of the high-affinity 8S-binding protein. In vivo estrogen treatment caused a time-related disappearance of the protein from the intact cytosol presumably as a result of translocation. No major differences in the degree of binding to the high-affinity component were observed in rats of different ages. Estrogens seem to exert direct effects on both stromal and epithelial tissues of the prostate.
ISSN:0148-5016
DOI:10.3109/01485018508986904