Regulation of corA , the Magnesium, Nickel, Cobalt Transporter, and Its Role in the Virulence of the Soft Rot Pathogen, Pectobacterium versatile Strain Ecc71
(formally ) causes disease on diverse plant species by synthesizing and secreting copious amount of plant-cell-wall-degrading exoenzymes including pectate lyases, polygalacturonases, cellulases, and proteases. Exoenzyme production and virulence are controlled by many factors of bacterial, host, and...
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Published in | Microorganisms (Basel) Vol. 11; no. 7; p. 1747 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
04.07.2023
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | (formally
) causes disease on diverse plant species by synthesizing and secreting copious amount of plant-cell-wall-degrading exoenzymes including pectate lyases, polygalacturonases, cellulases, and proteases. Exoenzyme production and virulence are controlled by many factors of bacterial, host, and environmental origin. The ion channel forming the magnesium, nickel, and cobalt transporter CorA is required for exoenzyme production and full virulence in strain Ecc71. We investigated CorA's role as a virulence factor and its expression in
.
. Inhibiting the transport function of CorA by growing a CorA
strain in the presence of specific CorA inhibitor, cobalt (III) hexaammine (Co (III)Hex), has no effect on exoenzyme production. Transcription of
, encoding a pectate lyase isozyme, is decreased in the absence of CorA, suggesting that CorA influences exoenzyme production at the transcriptional level, although apparently not through its transport function. CorA
and CorA
strains grown in the presence of Co (III)Hex transcriptionally express
at higher levels than CorA
strains in the absence of an inhibitor, suggesting the transport role of
contributes to autorepression. The expression of
is about four-fold lower in HrpL
strains lacking the
-specific extracytoplasmic sigma factor. The
promoter region contains a sequence with a high similarity to the consensus Hrp box, suggesting that
is part of Hrp regulon. Our data suggest a complex role, possibly requiring the physical presence of the CorA protein in the virulence of the
strain Ecc71. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2076-2607 2076-2607 |
DOI: | 10.3390/microorganisms11071747 |