Identification of P1 Variants of Mycoplasma pneumoniae by Use of High-Resolution Melt Analysis

• Published in: Journal of Clinical Microbiology Vol. 47; no. 12; pp. 4117 - 4120
• Main Authors: Schwartz, Stephanie B, Mitchell, Stephanie L, Thurman, Kathleen A, Wolff, Bernard J, Winchell, Jonas M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2009; American Society for Microbiology (ASM)
• Subjects: Adhesins, Bacterial - genetics; Bacterial Typing Techniques - methods; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genetic Variation; Genotype; Humans; Microbiology; Miscellaneous; Molecular Sequence Data; Mycoplasma pneumoniae; Mycoplasma pneumoniae - classification; Mycoplasma pneumoniae - genetics; Mycoplasma pneumoniae - isolation & purification; Pneumonia, Mycoplasma - epidemiology; Pneumonia, Mycoplasma - microbiology; Polymerase Chain Reaction - methods; Sequence Analysis, DNA; Transition Temperature; Mollicutes; Mycoplasmatales; Microbiology; Mycoplasma pneumoniae; Bacteria; Identification; Mycoplasmataceae
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.01696-09

Mycoplasma pneumoniae is a leading cause of community-acquired pneumonia. Although two genetically distinct types of M. pneumoniae are known, variants of each also exist. We used a real-time PCR high-resolution melt genotyping assay to identify clinical variants which may provide greater insight into the genetic distribution of M. pneumoniae strains.