In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers

guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in...

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Published inApplied physics letters Vol. 99; no. 16; p. 163701
Main Authors Yamamoto, Hideaki, Okano, Kazunori, Demura, Takanori, Hosokawa, Yoichiroh, Masuhara, Hiroshi, Tanii, Takashi, Nakamura, Shun
Format Journal Article
LanguageEnglish
Published United States 17.10.2011
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Summary:guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networks .
ISSN:0003-6951
DOI:10.1063/1.3651291