Characterization of Antibiotic Resistance in Escherichia coli Isolated from Shrimps and Their Environment

• Published in: Journal of food protection Vol. 77; no. 8; pp. 1394 - 1401
• Main Authors: CHANGKAEW, Kanjana, UTRARACHKIJ, Fuangfa, SIRIPANICHGON, Kanokrat, NAKAJIMA, Chie, SUTHIENKUL, Orasa, SUZUKI, Yasuhiko
• Format: Journal Article
• Language: English
• Published: Des Moines, IA International Association for Food Protection 01.08.2014; Elsevier Limited
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antimicrobial agents; Aquaculture; Bacteria; Biological and medical sciences; Decapoda - microbiology; Drug resistance; Drug Resistance, Bacterial; E coli; Environment; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - isolation & purification; Farms; Food contamination & poisoning; Food Contamination - analysis; Food Handling - instrumentation; Food industries; Food safety; Fundamental and applied biological sciences. Psychology; Genes; Integrons - genetics; Shellfish; Shellfish - microbiology; Shellfish farming; Thailand; Thailand; Escherichia coli; Macrura; Crustacea; Characterization; Resistance; Edible crustacean; Antibiotic; Arthropoda; Bacteria; Environment; Decapoda; Invertebrata; Shrimp; Enterobacteriaceae
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028X.JFP-13-510

Antimicrobial resistance in bacteria associated with food and water is a global concern. To survey the risk, 312 Escherichia coli isolates from shrimp farms and markets in Thailand were examined for susceptibility to 10 antimicrobials. The results showed that 17.6% of isolates (55 of 312) were resistant to at least one of the tested drugs, and high resistance rates were observed to tetracycline (14.4%; 45 of 312), ampicillin (8.0%; 25 of 312), and trimethroprim (6.7%; 21 of 312); 29.1% (16 of 55) were multidrug resistant. PCR assay of the tet (A), tet (B), tet (C), tet (D), tet (E), and tet (G) genes detected one or more of these genes in 47 of the 55 resistant isolates. Among these genes, tet (A) (69.1%; 38 of 55) was the most common followed by tet (B) (56.4%; 31 of 55) and tet (C) (3.6%; 2 of 55). The resistant isolates were further investigated for class 1 integrons. Of the 55 resistant isolates, 16 carried class 1 integrons and 7 carried gene cassettes encoding trimethoprim resistance (dfrA12 or dfrA17) and aminoglycosides resistance (aadA2 or aadA5). Two class 1 integrons, In54 (dfrA17-aadA5) and In27 (dfrA12-orfF-aadA2), were found in four and three isolates, respectively. These results indicate a risk of drug-resistant E. coli contamination in shrimp farms and selling places. The occurrence of multidrug-resistant E. coli carrying tet genes and class 1 integrons indicates an urgent need to monitor the emergence of drug-resistant E. coli to control the dissemination of drug-resistant strains and the further spread of resistance genes to other pathogenic bacteria.