Inactivation of bb0184, Which Encodes Carbon Storage Regulator A, Represses the Infectivity of Borrelia burgdorferi

• Published in: Infection and Immunity Vol. 79; no. 3; pp. 1270 - 1279
• Main Authors: Sze, Ching Wooen, Li, Chunhao
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.03.2011; American Society for Microbiology (ASM)
• Subjects: Acetyl Coenzyme A - metabolism; Adhesins, Bacterial - biosynthesis; Adhesins, Bacterial - genetics; Animals; Antigens, Bacterial - biosynthesis; Antigens, Bacterial - genetics; Bacterial Infections; Bacterial Outer Membrane Proteins - biosynthesis; Bacterial Outer Membrane Proteins - genetics; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Blotting, Western; Borrelia burgdorferi; Borrelia burgdorferi - genetics; Borrelia burgdorferi - metabolism; Borrelia burgdorferi - pathogenicity; Electrophoretic Mobility Shift Assay; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial - genetics; Mice; Mice, Inbred BALB C; Mice, SCID; Microbiology; Miscellaneous; Repressor Proteins - genetics; Repressor Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sigma Factor - biosynthesis; Sigma Factor - genetics; Signal Transduction - genetics; Spirochetes; Virulence - genetics; Virulence Factors - genetics; Virulence Factors - metabolism; Borrelia burgdorferi; Storage; Spirochaetaceae; Spirochaetales; Infectivity; Bacteria; Immunity
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00871-10

The genome of Borrelia burgdorferi, the Lyme disease spirochete, encodes a homolog (the bb0184 gene product) of the carbon storage regulator A protein (CsrABb); recent studies reported that CsrABb is involved in the regulation of several infectivity factors of B. burgdorferi. However, the mechanism involved remains unknown. In this report, a csrABb mutant was constructed and complemented in an infectious B31A3 strain. Subsequent animal studies showed that the mutant failed to establish an infection in mice, highlighting that CsrABb is required for the infectivity of B. burgdorferi. Western blot analyses revealed that the virulence-associated factors OspC, DbpB, and DbpA were attenuated in the csrABb mutant. The Rrp2-RpoN-RpoS pathway (σ⁵⁴-σS sigma factor cascade) is a central regulon that governs the expression of ospC, dbpB, and dbpA. Further analyses found that the level of RpoS was significantly decreased in the mutant, while the level of Rrp2 remained unchanged. A recent study reported that the overexpression of BB0589, a phosphate acetyl-transferase (Pta) that converts acetyl-phosphate to acetyl-coenzyme A (CoA), led to the inhibition of RpoS and OspC expression, suggesting that acetyl-phosphate is an activator of Rrp2. Along with this report, we found that CsrABb binds to the leader sequence of the bb0589 transcript and that the intracellular level of acetyl-CoA in the csrABb mutant was significantly increased compared to that of the wild type, suggesting that more acetyl-phosphate was being converted to acetyl-CoA in the mutant. Collectively, these results suggest that CsrABb may influence the infectivity of B. burgdorferi via regulation of acetate metabolism and subsequent activation of the Rrp2-RpoN-RpoS pathway.