Complete genome sequence and structural characterization of a novel iflavirus isolated from Opsiphanes invirae (Lepidoptera: Nymphalidae)

• Published in: Journal of invertebrate pathology Vol. 130; pp. 136 - 140
• Main Authors: Silva, Leonardo A., Ardisson-Araujo, Daniel M.P., Tinoco, Ricardo S., Fernandes, Odair A., Melo, Fernando L., Ribeiro, Bergmann M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2015
• Subjects: Animals; Base Sequence; Biological control; Elaeis guineensis; Genome sequencing; Genome, Viral - genetics; Iflavirus; Lepidoptera; Lepidoptera - virology; Microscopy, Electron, Transmission; Molecular Sequence Data; Nymphalidae; Opsiphanes invirae; Phylogeny; Picorna-like virus; Picornaviridae - physiology; Picornaviridae - ultrastructure; Spodoptera exigua; Iflavirus; Opsiphanes invirae; Genome sequencing; Biological control; Picorna-like virus
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2015.08.001

[Display omitted] •We characterized an iflavirus isolated from the palm oil lepidopterous pest, Opsiphanes invirae in Brazil.•This is the first report of iflavirus infecting O. invirae in Brazil.•This new virus (Opsiphanes invirae Iflavirus 1, OiIV-1) is more closely related to Spodoptera exigua iflavirus 1 (SeIV-1). Opsiphanes invirae (Lepidopera: Nymphalidae) is a common pest of the African oil palm tree (Elaeis guineensis) in Brazil. Dead larvae were collected in canopy of oil palm trees cultivated in the amazon region (Para State) and analyzed for viral infection. Electron microscopy of caterpillar extracts showed an icosahedral picorna-like virus particle with 30nm in diameter. Total RNA extracted from partially purified virus particles was sequenced. A contig of 10,083 nucleotides (nt) was identified and showed to encode one single predicted polyprotein with 3185 amino acid residues. Phylogenetic analysis showed that the new virus was closely related to another lepidopteran infective virus Spodoptera exigua iflavirus 1(SeIV-1), with 35% amino acid pairwise identity. The novel virus fulfils all ICTV requirements for a new iflavirus species and was named Opsiphanes invirae Iflavirus 1 (OilV-1).