Glucose metabolism sustains heme-induced Trypanosoma cruzi epimastigote growth in vitro

• Published in: PLoS neglected tropical diseases Vol. 17; no. 11; p. e0011725
• Main Authors: Silva Dias Vieira, Carolina, Pinheiro Aguiar, Ramon, de Almeida Nogueira, Natalia Pereira, Costa dos Santos Junior, Gilson, Paes, Marcia Cristina
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 01.11.2023; Public Library of Science (PLoS)
• Subjects: Acidification; Adenosine triphosphate; Blood; Care and treatment; Chagas disease; Diagnosis; Electron transport; Epimastigotes; Fermentation; Gene expression; Glucose; Glucose metabolism; Glycolysis; Health aspects; Heme; Insects; Metabolism; NMR; Nuclear magnetic resonance; Oxidants; Oxidative phosphorylation; Oxidizing agents; Oxygen consumption; Parasites; Phosphorylation; Physiology; Proliferation; Protozoa; Respiration; Transportation systems; Trypanosoma cruzi; Vector-borne diseases; Vertebrates; Brazil
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0011725

Chagas disease is caused by the protozoan parasite, Trypanosoma cruzi. This parasite alternates between an insect vector and a mammalian host. T. cruzi epimastigotes reside in the insect vector and coexist with the blood components of the vertebrate host. The metabolic profile of T. cruzi has been extensively studied; however, changes in its metabolism in response to signaling molecules present in the vector are poorly understood. Heme acts as a physiological oxidant that triggers intense epimastigote proliferation and upregulates the expression of genes related to glycolysis and aerobic fermentation in vitro. Here, heme-cultured epimastigotes increased D-glucose consumption. In fact, heme-cultured parasites secreted more succinate (the end product of the so-called succinic fermentation) followed by glucose intake. Increased succinate levels reduced the extracellular pH, leading to acidification of the supernatant. However, the acidification and proliferation stimulated by heme was impaired when glycolysis was inhibited. Otherwise, when glucose amount is enhanced in supernatant, heme-cultured parasites increased its growth whereas the glucose depletion caused a delay in proliferation. Heme supplementation increased epimastigote electron transport system-related O.sub.2 consumption rates, while glucose addition reduced both the electron transport system-related O.sub.2 consumption rates and spare respiratory capacity, indicating a Crabtree-like effect. These results show that glycolysis predominated in heme-cultured epimastigotes over oxidative phosphorylation for energy supply when glucose is present to sustain its high proliferation in vitro. Furthermore, it provided an insight into the parasite biology in the vector environment that supply glucose and the digestion of blood generates free heme that can lead to the growth of T. cruzi epimastigotes.