Nutrient Acquisition and Population Growth of a Mixotrophic Alga in Axenic and Bacterized Cultures

• Published in: Microbial ecology Vol. 42; no. 4; pp. 513 - 523
• Main Authors: Sanders, R. W., D. A. Caron, Davidson, J. M., M. R. Dennett, D. M. Moran
• Format: Journal Article
• Language: English
• Published: New York, NY Springer-Verlag New York Inc 01.12.2001; Springer
• Subjects: Algae; Animal, plant and microbial ecology; Axenic culture; Bacteria; Biological and medical sciences; Biomass production; Freshwater; Fundamental and applied biological sciences. Psychology; Ingestion; Marine; Microbial ecology; Microflora of plants; Nutrients; Nutrition; Ochromonas; Organic foods; Phosphorus; Salts; Various environments (extraatmospheric space, air, water); Population growth; Growth rate; Growth; Algae; Biomass; Glucose; Acquisition; Substrate; Light; Bacteria; Nutrient; Thallophyta; Ecological abundance; Organic compounds
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-001-1024-6

Axenic growth of a mixotrophic alga, Ochromonas sp., was compared in several inorganic and organic media, and in the presence of live bacteria under nutrient-replete and low-nutrient conditions. Axenic growth in the light was negligible in inorganic media with or without the addition of glucose. Addition of vitamins increased growth rate, but average cell size declined, resulting in no net increase in biomass. Supplementing axenic cultures with a more complex organic substrate resulted in moderate growth and higher maximal abundance (and biomass) than in the inorganic media with added vitamins. The absence of light did not greatly affect population growth rate in the presence of complex dissolved organic compounds, although cell size was significantly greater in the light than in the dark. The highest growth rates for the alga (up to 2.6 d-1) were measured in treatments containing live bacteria. Increases in cell number of Ochromonas sp. in the presence of bacterial prey were similar in the light and dark, although chloroplast and cell sizes differed. Bacterial abundance was reduced and dissolved phosphorus and ammonia were rapidly released in bacterized cultures in the light and dark, indicating high rates of bacterial ingestion and suggesting an inability of the alga to store or utilize N and P in excess of the quantities required for heterotrophic growth. Low-nutrient conditions in the presence of bacteria were promoted by adding glucose to stimulate bacterial growth and the uptake of N and P released by algal phagotrophy. Subsequent decreases in dissolved N and P following the addition of glucose corresponded to a second period of rapid growth of the alga in both light and dark. This result, combined with evidence for slow axenic growth of this strain, indicated that nutrient acquisition for this species in the presence of bacteria was accomplished primarily via ingestion of bacteria.