New Member of Family 11 Polysaccharide Lyase, Rhamnogalacturonan Lyase (CtRGLf) from Clostridium thermocellum

• Published in: Molecular biotechnology Vol. 58; no. 4; pp. 232 - 240
• Main Authors: Dhillon, Arun, Fernandes, Vania O., Dias, Fernando M. V., Prates, José A. M., Ferreira, Luis M. A., Fontes, Carlos M. G. A., Centeno, M. S. J., Goyal, Arun
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2016; Springer Nature B.V
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biochemistry; Biological Techniques; Biotechnology; Calcium - metabolism; Catalysis; Cell Biology; Chemistry; Chemistry and Materials Science; Cloning, Molecular; Clostridium thermocellum; Clostridium thermocellum - enzymology; Clostridium thermocellum - genetics; Enzymatic activity; Enzymes; Flowers & plants; Human Genetics; Original Paper; Pectins - metabolism; Polysaccharide-Lyases - genetics; Polysaccharide-Lyases - metabolism; Protein Science; Substrate Specificity; Rhamnogalacturonan; Pectin; Rhamnogalacturonan oligosaccharides; Rhamnogalacturonan Lyase; Polysaccharide lyase; Pectic oligosaccharides; Clostridium thermocellum
• ISSN: 1073-6085; 1559-0305
• DOI: 10.1007/s12033-016-9921-6

A thermostable, alkaline rhamnogalacturonan lyase (RG lyase) CtRGLf, of family 11 polysaccharide lyase from Clostridium thermocellum was cloned, expressed, purified and biochemically characterised. Both, the full-length CtRGLf (80 kDa) protein and its truncated derivative CtRGL (63.9 kDa) were expressed as soluble proteins and displayed maximum activity against rhamnogalacturonan I (RG I). CtRGLf showed maximum activity at 70 °C, while CtRGL at 60 °C. Both enzymes showed maximum activity at pH 8.5. CtRGLf and CtRGL do not show higher activity on substrates with high β-D-galactopyranose (D-Galp) substitution, this catalytic property deviates from that of some earlier characterised RG lyases which prefer substrates with high D-Galp substitution. The enzyme activity of CtRGLf and CtRGL was enhanced by 1.5 and 1.3 fold, respectively, in the presence of 3 mM of Ca²⁺ ions. The TLC analysis of the degraded products of RG I, released by the action of CtRGLf and CtRGL revealed the production of RG oligosaccharides as major products confirming their endolytic activity.