Angiotensin II induces formation of the early growth response gene-1 protein in rat vascular smooth muscle cells

• Published in: FEBS letters Vol. 313; no. 2; pp. 109 - 112
• Main Authors: Sachinidis, Agapios, Weisser, Peter, Ko, Yon, Schulte, Kerstin, zu Brickwedde, Katharina Meyer, Neyses, Ludwig, Vetter, Hans
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23.11.1992; Elsevier
• Subjects: Ang II; Angiotensin II; Angiotensin II - pharmacology; Animals; Biological and medical sciences; Cells, Cultured; DMEM; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - genetics; Dulbecco's modified Eagles medium; Dulbecco's phosphate-buffered saline; Early growth responce gene-1 (Egr-1); early growth response gene-1; Early Growth Response Protein 1; Egr-1; Female; Fundamental and applied biological sciences. Psychology; Gene expression; Immediate-Early Proteins; Molecular and cellular biology; Molecular genetics; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; PBS; Phosphatidylinositols - metabolism; Rats; Rats, Inbred WKY; RNA, Messenger - analysis; Transcription Factors - biosynthesis; Transcription Factors - genetics; Vascular smooth muscle cell; vascular smooth muscle cells; VSMCs; PBS; DMEM; VSMCs; Egr-1; Vascular smooth muscle cell; Angiotensin II; Early growth responce gene-1 (Egr-1); Ang II; Vertebrata; Phosphoinositide; Mammalia; Messenger RNA; Rat; Rodentia; Peptide hormone; Turnover; Smooth muscle; Gene expression; Transcription factor
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)81423-J

The effect of angiotensin II (Ang II) on the early growth response gene-1 (Egr-1) mRNA, on the Egr-1 protein and on the phosphoinositide PI turnover signalling system was investigated in the presence and absence of EXP3174, a potent non-peptide Ang II receptor antagonist. Ang II induced an accumulation of 3.4 kb Egr-1 mRNA and the 80 kDa Egr-1 protein, with a maximum at 30 min and 60 min, respectively. EXP3174 blocked the Ang II-induced increase of inositolphosphates, Egr-1 mRNA and the Egr-1 protein, suggesting the involvement of the PI signalling system by the expression of the Egr-1 gene.