d-Glyceraldehyde-3-phosphate dehydrogenase purified from rabbit muscle contains phosphotyrosine

• Published in: FEBS letters Vol. 304; no. 1; pp. 21 - 23
• Main Authors: Sergienko, Edward A., Kharitonenkov, Alexey I., Bulargina, Tamara V., Muronetz, Vladimir V., Nagradova, Natalia K.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 08.06.1992; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Antibody to phosphotyrosine; Biological and medical sciences; d-Glyceraldehyde-3-phosphate dehydrogenase; Endogenous phosphate; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Glyceraldehyde-3-Phosphate Dehydrogenases - chemistry; Glyceraldehyde-3-Phosphate Dehydrogenases - isolation & purification; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism; Immunoblotting; Muscles - enzymology; Oxidoreductases; Phosphorylation; Phosphotyrosine; Rabbits; Tyrosine - analogs & derivatives; Tyrosine - analysis; Endogenous phosphate; Antibody to phosphotyrosine; d-Glyceraldehyde-3-phosphate dehydrogenase; Phosphotyrosine; Site specificity; Vertebrata; Phosphorylation; Mammalia; Enzyme; Glyceraldehyde-phosphate dehydrogenase; Rabbit; Lagomorpha; Striated muscle
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)80580-A

Homogeneous preparations of d-glyceraldehyde-3-phosphate dehydrogenase purified from rabbit muscle were found to contain 0.2–0.7 moles of covalently bound phosphate per mole of the enzyme. With the use of anti-phosphotyrosine antibodies, evidence was obtained that the enzyme is phosphorylated at tyrosine residues.