Performance of the VERSANT ® HIV-1 Resistance Assays (LiPA) for detecting drug resistance in therapy-naive patients infected with different HIV-1 subtypes

• Published in: FEMS immunology and medical microbiology Vol. 39; no. 2; pp. 119 - 124
• Main Authors: Derdelinckx, Inge, Van Laethem, Kristel, Maes, Bart, Schrooten, Yoeri, De Schouwer, Kirsten, De Wit, Stéphane, Fransen, Katrien, Garcı́a Ribas, Sergio, Moutschen, Michel, Vaira, Dolores, Zissis, Georges, Van Ranst, Marc, Van Wijngaerden, Eric, Vandamme, Anne-Mieke
• Format: Journal Article Web Resource
• Language: English
• Published: Oxford, UK Elsevier B.V 28.11.2003; Blackwell Publishing Ltd; Elsevier Science Bv
• Subjects: Anti-HIV Agents - pharmacology; Anti-HIV Agents - therapeutic use; Belgium - epidemiology; Drug Resistance, Viral - genetics; Female; Genotype; HIV Infections - drug therapy; HIV Infections - virology; HIV Protease - genetics; HIV Reverse Transcriptase - genetics; HIV-1 - classification; HIV-1 - drug effects; HIV-1 - genetics; HIV-1 - isolation & purification; Human health sciences; Human immunodeficiency virus type 1; Humans; Immunologie & maladie infectieuse; Immunology & infectious disease; Line probe assay; Male; Microbial Sensitivity Tests - methods; Mutation; Nucleic Acid Hybridization; Resistance testing; Sciences de la santé humaine; Sequence Analysis, DNA - methods; Subtypes; Belgium; Human immunodeficiency virus type 1; Subtypes; Line probe assay; Resistance testing
• ISSN: 0928-8244; 1574-695X
• DOI: 10.1016/S0928-8244(03)00240-2

In this study we evaluated the performance of the VERSANT ® HIV-1 Resistance Assays (LiPA) in detecting drug resistance in therapy-naive HIV-infected patients diagnosed in Belgium in 2000. We compared the results with population sequencing and found concordance to be in line with previous studies in treatment-experienced patients (86.87% for reverse transcriptase (RT); 92.77% for protease (PRO)). Discordance was mainly due to indeterminate reactions on LiPA (8.45% for RT; 6.85% for PRO) and minor discordances (4.13% for RT; 0.25% for PRO). Major discordances were rare (0.46% for RT; 0.12% for PRO). Indeterminate reactions were significantly associated with strains belonging to non-B subtypes.