The intriguing links between prominin-1 (CD133), cholesterol-based membrane microdomains, remodeling of apical plasma membrane protrusions, extracellular membrane particles, and (neuro)epithelial cell differentiation

• Published in: FEBS letters Vol. 584; no. 9; pp. 1659 - 1664
• Main Authors: Corbeil, Denis, Marzesco, Anne-Marie, Wilsch-Bräuninger, Michaela, Huttner, Wieland B.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 03.05.2010
• Subjects: AC133 Antigen; Animals; Antigens, CD - chemistry; Antigens, CD - physiology; Cell Differentiation - physiology; Cell Membrane - chemistry; Cell Membrane - metabolism; Cell Surface Extensions - chemistry; Cell Surface Extensions - metabolism; Cell-Derived Microparticles - metabolism; Cell-Derived Microparticles - physiology; Cholesterol; Cholesterol - chemistry; Cholesterol - metabolism; Epithelial Cells - metabolism; Epithelial Cells - physiology; Extracellular Space - metabolism; Glycoproteins - chemistry; Glycoproteins - physiology; Humans; Membrane Microdomains - chemistry; Membrane Microdomains - metabolism; Membrane polarity; Microvilli; Models, Biological; Neurons - metabolism; Neurons - physiology; Peptides - chemistry; Peptides - physiology; Primary cilium; Prominin-1; Primary cilium; Prominin-1; Membrane polarity; Cholesterol; Microvilli
• ISSN: 0014-5793; 1873-3468; 1873-3468
• DOI: 10.1016/j.febslet.2010.01.050

Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane protein concentrated in plasma membrane protrusions. In epithelial cells, notably neuroepithelial stem cells, prominin-1 is found in microvilli, the primary cilium and the midbody. These three types of apical membrane protrusions are subject to remodeling during (neuro)epithelial cell differentiation. The protrusion-specific localization of prominin involves its association with a distinct cholesterol-based membrane microdomain. Moreover, the three prominin-1-containing plasma membrane protrusions are the origin of at least two major subpopulations of prominin-1-containing extracellular membrane particles. Intriguingly, the release of these particles has been implicated in (neuro)epithelial cell differentiation.