Na +-coupled D-glucose uptake and membrane order of enterocyte brush border membrane vesicles, under the effect of a series of N-phenylcarbamates

• Published in: FEBS letters Vol. 201; no. 1; pp. 119 - 123
• Main Authors: Fernandez, Y., Boigegrain, R.-A., Cambon-Gros, C., Deltour, P., Mitjavila, S.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 26.05.1986; Elsevier
• Subjects: Animals; Biological and medical sciences; Biological Transport - drug effects; Carbamates - pharmacology; Chlorpropham - pharmacology; ESR N-Phenylcarbamate; Glucose - metabolism; Glucose transport; Hydrophobic effect; Intestine, Small - drug effects; Intestine, Small - metabolism; Kinetics; Lipid Bilayers; Medical sciences; Membrane fluidity; Membrane Fluidity - drug effects; Microvilli - metabolism; Pesticides, fertilizers and other agrochemicals toxicology; Phenylcarbamates; Rat intestinal brush border; Rats; Sodium - metabolism; Toxicology; Hydrophobic effect; Membrane fluidity; Rat intestinal brush border; ESR N-Phenylcarbamate; Glucose transport; Brush border; Toxicity; Pesticides; Enterocyte; Urethane; Glucose; Metabolism; In vitro; Uptake; Herbicide
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)80582-8

The importance of the hydrophobic effect of exogenous substances and of modifications of membrane order on D-glucose uptake are still poorly defined. Our results show that the concentrative Na + -coupled D-glucose uptake of rat enterocyte brush border membrane vesicles is inhibited by N-phenylcarbamate derivatives. Unlike other series of lipophilic compounds inhibiting D-glucose transport, these N-phenylcarbamates increase the membrane order. However, since the concentrations required for membrane order increase are much greater than those active on D-glucose uptake, the effects on lipid order cannot be resposible for the inhibition of D-glucose uptake. Measurements of D-glucose uptake under conditions of Na + equilibrium show that these carbamates do not act directly on the carrier but indirectly by favouring the dissipation of the Na + gradient.