Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: allelic polymorphism and degree of relationship
Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The numb...
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Published in | American journal of botany Vol. 87; no. 11; pp. 1647 - 1655 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Botanical Soc America
01.11.2000
Botanical Society of America Botanical Society of America, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The number of alleles per locus ranged from four to 21 alleles, and allelic diversity was greater in the wild species than in cassava. Seventy-nine alleles, including unique ones, were detected in the wild species but were not found in the crop. The lower level of heterozygosity in some wild species probably resulted from a combination of fine-scale differentiation within the species and the presence of null alleles. Overall, microsatellite primers worked across the genus, but, with increasing genetic distance, success in amplifying loci tended to decrease. No accession of M. aesculifolia, M. carthaginensis, and M. brachyloba presented a banding pattern at locus Ga-140; neither did one appear for M. aesculifolia at locus Ga-13. Previous work with amplified fragment length polymorphism (AFLP) markers and this microsatellite analysis show that these three wild taxa are the most distant relatives of the crop, whereas the wild forms M. esculenta subsp. flabellifolia and M. esculenta subsp. peruviana appear to be the closest. |
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Bibliography: | The authors thank Dr. Morry Levy, Purdue University, for his statistical advice on microsatellite analysis, Dr. Gary Kochert, University of Georgia, for generously providing the cassava microsatellite primers, and Elizabeth de Paéz for editorial assistance. This research was supported in part by a grant from the Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología “Francisco José de Caldas” (COLCIENCIAS). j.tohme@cgiar.org Current address: Purdue University, Department of Biological Sciences, Lilly Hall, Room G‐420A, West Lafayette, Indiana 47907–1392 USA. Current address: Weaver Popcorn NC, 1000 North 325 W, PO Box 207, New Richmond, Indiana 47967 USA. Current address: Centro Internacional de la Papa‐CIP, Apartado 1558, Lima 12, Peru. Auther for reprint requests (Tel.: ++57‐2‐4450000 ext. 3265; fax: ++57‐2‐4450073; e‐mail . Current address: Center for the Application of Molecular Biology to International Agriculture‐CAMBIA GPO Box 3200, Canberra, ACT 2601, Australia. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0002-9122 1537-2197 |
DOI: | 10.2307/2656741 |