A model for studying epithelial attachment and morphology at the interface between skin and percutaneous devices
Percutaneous devices are indispensable in modern medicine, yet complications from their use result in significant morbidity, mortality, and cost. Bacterial biofilm at the device exit site accounts for most infections in short‐term devices. We hypothesize that advanced biomaterials can be developed t...
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Published in | Journal of biomedical materials research. Part A Vol. 74A; no. 3; pp. 482 - 488 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.09.2005
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Subjects | |
Online Access | Get full text |
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Summary: | Percutaneous devices are indispensable in modern medicine, yet complications from their use result in significant morbidity, mortality, and cost. Bacterial biofilm at the device exit site accounts for most infections in short‐term devices. We hypothesize that advanced biomaterials can be developed that facilitate attachment of skin cells to percutaneous devices, forming a seal to preclude bacterial invasion. To study the skin/biomaterial interface systematically, we first identified biomaterials with physical properties compatible with histological processing of skin. Second, we developed an organ culture system to study skin response to implants. Organ cultures implanted with porous poly(2‐hydroxyethyl methacrylate) [poly(HEMA)] or polytetrafluoroethylene (PTFE) could easily be evaluated histologically with preservation of the skin/biomaterial interface. Epithelial cells migrated down the cut edges of the biomaterial in a pattern seen in marsupialization of percutaneous devices in vivo. This in vitro model maintains skin viability and allows histologic evaluation of the skin/biomaterial interface, making this a useful, inexpensive test‐bed for studies of epidermal attachment to modified biomaterials. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005 |
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Bibliography: | NSF - No. EEC 9529161 ark:/67375/WNG-N9VCH91L-8 Marvin and Judy Young Research Fund istex:6B6D9A2BB7E1789B7FF7A9B721218F3CD4AC6F15 ArticleID:JBM30384 NIH - No. DK59221 The George F. Odland Endowed Research Fund ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1549-3296 1552-4965 |
DOI: | 10.1002/jbm.a.30384 |