Chromatin texture changes related to proliferation and maturation in erythrocytes

• Published in: Cytometry (New York, N.Y.) Vol. 9; no. 4; pp. 339 - 348
• Main Authors: Giroud, Françloise, Gauvain, Catherine, Seigneurin, Daneil, Hagen, Victoria Von
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.07.1988; Wiley-Liss
• Subjects: Biological and medical sciences; Bone Marrow Cells; Cell Cycle; Cell cycle analysis; Cell Differentiation; Cell differentiation, maturation, development, hematopoiesis; Cell Division; Cell physiology; Chromatin - analysis; differentiation in erythroblastic lineage; DNA - analysis; Erythroblasts - analysis; Erythroblasts - cytology; Erythrocytes - analysis; Erythrocytes - cytology; Erythropoiesis; Fundamental and applied biological sciences. Psychology; Humans; Molecular and cellular biology; Staining and Labeling; Erythropoiesis; Cell proliferation; Chromatin; Erythroid cell; Cell cycle; Cell differentiation; Erythroblast
• ISSN: 0196-4763; 1097-0320
• DOI: 10.1002/cyto.990090411

Erythroid cell types were quantitatively assessed on the basis of the analysis of their nuclear images after Feulgen and Heidenhain blue counterstaining. An original nonsupervised classification method, developed in our laboratory, was applied to cell cycle analysis. Results obtained suggest that, in the four proliferative generation cycles, which concur with the successive morphological stages, there is a sequential flow of cells from one morphological stage to the next by cell cycling. We show that the chromatin pattern alone made it possible to set up a metric of the whole differentiation process occurring in the normal human erythroblastic lineage in which proliferating events occur.