A large-scale multimodal investigation of the interplay between the serotonergic system and emotion processing

• Published in: Translational psychiatry Vol. 15; no. 1; pp. 196 - 10
• Main Authors: Klöbl, Manfred, Murgaš, Matej, Reed, Murray Bruce, Silberbauer, Leo Robert, Hartmann, Annette M., Godbersen, Godber Mathis, Gryglewski, Gregor, Nics, Lukas, Hahn, Andreas, Rujescu, Dan, Hacker, Marcus, Lanzenberger, Rupert
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.06.2025; Nature Publishing Group
• Subjects: 59/36; 59/78; 631/378/340; 692/699/476/1414; Adult; Behavioral Sciences; Biological Psychology; Citalopram - pharmacology; Depressive Disorder, Major - diagnostic imaging; Depressive Disorder, Major - drug therapy; Depressive Disorder, Major - genetics; Depressive Disorder, Major - metabolism; Depressive Disorder, Major - physiopathology; Depressive Disorder, Major - psychology; Emotions; Emotions - drug effects; Emotions - physiology; Facial Expression; Facial Recognition - drug effects; Facial Recognition - physiology; Female; Gyrus Cinguli - diagnostic imaging; Gyrus Cinguli - drug effects; Gyrus Cinguli - physiopathology; Humans; Magnetic Resonance Imaging; Male; Medicine; Medicine & Public Health; Mental depression; Middle Aged; Neurosciences; Pharmacotherapy; Positron-Emission Tomography; Psychiatry; Selective Serotonin Reuptake Inhibitors - pharmacology; Serotonin - metabolism; Serotonin Plasma Membrane Transport Proteins - genetics; Serotonin Plasma Membrane Transport Proteins - metabolism; Tranquilizers; Young Adult
• ISSN: 2158-3188; 2158-3188
• DOI: 10.1038/s41398-025-03407-2

Considering the complexity of serotonergic influence on emotions, we conducted a comprehensive investigation of the interplay between emotion processing and the serotonergic system using simultaneous functional and molecular neuroimaging during pharmacological challenge while disentangling the effects of serotonin transporter (SERT) binding, genotype, and diagnosis of major depressive disorder (MDD). Herein, 153 subjects (44 with MDD) performed a facial emotion processing task during functional magnetic resonance imaging (fMRI) before and after an acute intravenous application of 8 mg citalopram or placebo. Patients with MDD were assessed again after at least three months of antidepressant treatment. Citalopram administration resulted in a reduced fMRI activation in regions involved in fear processing, including the anterior cingulate cortex (ACC), when viewing fearful faces contrasted against happy or neutral faces. ACC activation correlated negatively with striatal/thalamic SERT availability across drug conditions as measured by [11 C]DASB positron emission tomography. Across groups, citalopram-induced changes in ACC activation correlated with emotional attribution, indicating stronger reductions for subjects with higher self- versus other- attribution. Moreover, striatal SERT availability mediated the influence of the number of 5-HTTLPR/rs25531 L A alleles on ACC activation under placebo. Patients with MDD exhibited increased activations in the intraparietal and superior frontal sulcus in response to fearful versus happy faces at baseline, and along the parieto-occipital/calcarine fissure after treatment. We interpret our findings on multiple levels of the serotonergic-emotional interaction within the context of enhanced passive coping and acute anxiolytic effects of citalopram following potential changes in serotonin or SERT availability.