Overexpression, Purification, Characterization, and Pathogenicity of Vibrio harveyi Hemolysin VHH

Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, a...

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Published inInfection and Immunity Vol. 74; no. 10; pp. 6001 - 6005
Main Authors Zhong, Yingbin, Zhang, Xiao-Hua, Chen, Jixiang, Chi, Zhenghao, Sun, Boguang, Li, Yun, Austin, Brian
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.10.2006
Subjects
Amino Acid Sequence
Animals
Bacterial Proteins - chemistry
Bacterial Proteins - isolation & purification
Bacterial Proteins - toxicity
Bacteriology
Base Sequence
Biological and medical sciences
Escherichia coli
Escherichia coli - genetics
Flounder - microbiology
Fundamental and applied biological sciences. Psychology
Hemolysin Proteins - chemistry
Hemolysin Proteins - isolation & purification
Hemolysin Proteins - toxicity
Microbiology
Miscellaneous
Molecular Pathogenesis
Molecular Sequence Data
Phospholipases - chemistry
Phospholipases - isolation & purification
Phospholipases - toxicity
Pleuronectiformes
Temperature
Vibrio - enzymology
Vibrio harveyi
Pathogenicity
Purification
Microbiology
Vibrio harveyi
Bacteria
Vibrionaceae
Hemolysin
Immunity
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Summary:Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.
Bibliography:http://iai.asm.org/
ObjectType-Article-1
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Editor: V. J. DiRita
Corresponding author. Mailing address: Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China. Phone and fax: 0086-532-82032266. E-mail: xhzhang@ouc.edu.cn.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.00512-06