Novel Device That Produces Carbon Dioxide Mist for Myocardial Infarction Treatment in Rats

• Published in: Circulation Journal Vol. 76; no. 5; pp. 1203 - 1212
• Main Authors: Yamazaki, Takanori, Izumi, Yasukatsu, Nakamura, Yasuhiro, Hanatani, Akihisa, Shimada, Kenei, Muro, Takashi, Shiota, Masayuki, Iwao, Hiroshi, Yoshiyama, Minoru
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Circulation Society 2012
• Subjects: Animal model; Animals; Carbon dioxide; Carbon Dioxide - pharmacology; Echocardiography; Endothelial Growth Factors - metabolism; Injections, Subcutaneous - instrumentation; Injections, Subcutaneous - methods; Male; Mist; Myocardial infarction; Myocardial Infarction - diagnostic imaging; Myocardial Infarction - drug therapy; Myocardial Infarction - metabolism; Myocardial Infarction - physiopathology; Oxyhemoglobins - metabolism; Rats; Rats, Wistar; Stroke Volume - drug effects; Time Factors; Water
• ISSN: 1346-9843; 1347-4820
• DOI: 10.1253/circj.CJ-11-1116

Background: Carbon dioxide (CO2) baths have been used to treat a variety of diseases, but developing an artificial bath of highly concentrated CO2 is difficult. Here, we tried the efficacy of a novel device instead of a CO2 bath. Methods and Results: Using a device equipped with double fluid nozzles, CO2 gas and H2O were compounded and compressed at 4 barometric pressures. As a result, CO2 gas was dissolved in H2O, which contained a few micrometers of CO2 particles, namely, a CO2 mist. Wistar rats with myocardial infarction (MI) by ligation of the left coronary artery were percutaneously administered CO2 mist or CO2 gas alone or no treatment for 30min daily. With regard to tissue blood flow during treatment, the group treated with CO2 mist had significantly increased tissue oxygenated hemoglobin levels and tissue saturation levels, and significantly decreased deoxygenated hemoglobin levels compared with the group treated with CO2 gas. After 4 weeks treatment, the group treated with CO2 mist had a significantly improved ejection fraction by echocardiography compared with the untreated group. Interestingly, the group treated with CO2 mist had significantly increased nitrate concentrations in serum and vascular endothelial growth factor mRNA expression levels in the myocardium compared with the untreated group. Conclusions: Our new mist production device may be potentially useful for the treatment of heart failure caused by MI. (Circ J 2012; 76: 1203-1212)