Evaluation of the areas of neuronal cell bodies and nuclei in the myenteric plexus of the duodenum of adult rats

• Published in: Arquivos de neuro-psiquiatria Vol. 58; no. 2A; pp. 246 - 251
• Main Authors: Miranda-Neto, M H, Furlan, M M, Sant'Ana, D D, Molinari, S L, Souza, J A
• Format: Journal Article
• Language: English
• Published: Brazil Academia Brasileira de Neurologia - ABNEURO 01.06.2000; Academia Brasileira de Neurologia (ABNEURO)
• Subjects: Animals; Cell Nucleus; Cell Size; duodenum; Duodenum - cytology; Duodenum - innervation; Male; myenteric neurons; Myenteric Plexus - cytology; neuronal area; Neurons - cytology; NEUROSCIENCES; PSYCHIATRY; Rats; Rats, Wistar; duodeno; área neuronal; rats; neuronal area; ratos; myenteric neurons; duodenum; neurônios mientéricos
• ISSN: 0004-282X; 1678-4227; 0004-282X; 1678-4227
• DOI: 10.1590/S0004-282X2000000200007

This study compared the areas of cell body and nucleus profiles of the myenteric neurons in the antimesenteric and intermediate regions of the duodenum of adult rats. Five male rats were used. The duodenum was removed and dissected to whole-mount preparations, which were stained by the Giemsa technique. The areas of cell body and nucleus profiles of 100 neurons, 50 from each region, of each animal, were assessed with image analyser. Based on the global mean+/-SD of the areas of cell body profiles, neurons were labelled as small, medium or large. It was observed that the neurons did not differ significantly in size or incidence between the antimesenteric and intermediate regions. However, the nuclei of the small and medium neurons were significantly smaller in the latter region. It is discussed that the smaller nuclear size could be related to the cell bodies being slightly smaller on this region and to a possible smaller biosynthetic activity which would influence nuclear size.