Prevalence and Characterization of Salmonella Isolated from Chickens in Anhui, China

• Published in: Pathogens (Basel) Vol. 12; no. 3; p. 465
• Main Authors: Shen, Xuehuai, Yin, Lei, Zhang, Anyun, Zhao, Ruihong, Yin, Dongdong, Wang, Jieru, Dai, Yin, Hou, Hongyan, Pan, Xiaocheng, Hu, Xiaomiao, Zhang, Danjun, Liu, Yongjie
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.03.2023; MDPI
• Subjects: antibiotic resistance genes; Antibiotics; Antimicrobial agents; Antimicrobial resistance; antimicrobial susceptibility; Bacterial infections; biofilm formation; Biofilms; chicken; Chickens; Doxycycline; Drug resistance; Embryos; Epidemiology; Genes; Genomes; Imipenem; Infections; Multidrug resistance; Penicillin; Phenotypes; Polymyxin B; Poultry; Public health; Salmonella; serovar; Sulfonamides; Tetracyclines; Virulence; Beijing China; Austria; Anhui China; China; Salmonella; multilocus sequence typing; chicken; antimicrobial susceptibility; serovar; biofilm formation; antibiotic resistance genes; virulence genes
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens12030465

is one of the most important zoonotic pathogens that can cause both acute and chronic illnesses in poultry flocks, and can also be transmitted to humans from infected poultry. The purpose of this study was to investigate the prevalence, antimicrobial resistance, and molecular characteristics of isolated from diseased and clinically healthy chickens in Anhui, China. In total, 108 isolates (5.66%) were successfully recovered from chicken samples ( = 1908), including pathological tissue (57/408, 13.97%) and cloacal swabs (51/1500, 3.40%), and Enteritidis (43.52%), Typhimurium (23.15%), and Pullorum (10.19%) were the three most prevalent isolates. isolates showed high rates of resistance to penicillin (61.11%), tetracyclines (47.22% to tetracycline and 45.37% to doxycycline), and sulfonamides (48.89%), and all isolates were susceptible to imipenem and polymyxin B. In total, 43.52% isolates were multidrug-resistant and had complex antimicrobial resistance patterns. The majority of isolates harbored (77.78%), (61.11%), and (63.89%) genes, and the antimicrobial resistance genes in the isolates were significantly positively correlated with their corresponding resistance phenotype. isolates carry high rates of virulence genes, with some of these reaching 100% ( , , and ). Fifty-seven isolates (52.78%) were biofilm-producing. The 108 isolates were classified into 12 sequence types (STs), whereby ST11 (43.51%) was the most prevalent, followed by ST19 (20.37%) and ST92 (13.89%). In conclusion, infection in chicken flocks is still serious in Anhui Province, and not only causes disease in chickens but might also pose a threat to public health security.