Polyamine biosynthesis regulated by StARD expression plays an important role in potato wound periderm formation

• Published in: Plant and cell physiology Vol. 49; no. 10; pp. 1627 - 1632
• Main Authors: Kim, J.H.(Korea Research Inst. of Bioscience and Biotechnology, Daejon (Korea R.)), Kim, H.S, Lee, Y.H, Kim, Y.S, Oh, H.W, Joung, H, Chae, S.K, Suh, K.H, Jeon, J.H
• Format: Journal Article
• Language: English
• Published: Japan Oxford University Press 01.10.2008; Oxford Publishing Limited (England)
• Subjects: ARD; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Dioxygenases - metabolism; EXPRESION GENICA; Expressed Sequence Tags; EXPRESSION DES GENES; GENE; GENE EXPRESSION; Gene Expression Regulation, Plant; Gene Library; GENES; HERIDAS; Lipids - biosynthesis; Methionine - metabolism; Methionine salvage; PCR; PERIDERM; PERIDERME; PERIDERMIS; PLAIE; Plant Tubers - genetics; Plant Tubers - metabolism; POLIAMINAS; POLYAMINE; POLYAMINES; Polyamines - metabolism; Potato; Reverse Transcriptase Polymerase Chain Reaction; RNA, Plant - genetics; SOLANUM TUBEROSUM; Solanum tuberosum - enzymology; Solanum tuberosum - genetics; Suberization; TRANSCRIPCION; TRANSCRIPTION; WOUNDS; Methionine salvage; Potato; Polyamine; ARD; Suberization
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcn115

An acireductone dioxygenase (ARD) gene of potatoes was isolated from the expressed sequence tags (ESTs) of potato post-suberization cDNA libraries. The highest expression levels of the StARD gene and the protein appeared 36 h after suberization. An approximate 9-fold increase in ARD activity was detected at 36 h after wounding. Real-time reverse transcription-PCR (RT-PCR) analysis and immunolocalization studies revealed that StARD transcripts increase at the wound surface of potato tubers. The polyamine (PA) contents increased significantly after wounding at the wound surface. The increased PA content and ARD activity may play an important role in wound periderm formation.